Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- GTX11927 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX11927, RRID:AB_368072
- Product name
- D4 GDI (cleavage specific) antibody [97A1015]
- Antibody type
- Monoclonal
- Reactivity
- Human, Mouse
- Host
- Mouse
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Supportive validation
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- GeneTex (provider)
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- Experimental details
- Western blot analysis of D4GDI in Jurkat cells using at 2 μg/ml and IMG-160A at 0.1 μg/ml. Cells were treated with 2 uM staurosporine for different time periods. IMG-396A detects both the full-length and cleaved forms of D4GDI while IMG-160A specifically detects only the cleaved form of the protein. IMGENEX's goat anti-mouse Ig HRP conjμgate (IMGENEX) was used for this test.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- HL60 cells were left untreated (green) or treated with camptothecin to induce apoptosis (red). Cells were analyzed by intracellular flow cytometry using either the or D4-GDI antibodies (0.5 μg antibody/test/1 million cells) and Imgenex's Intracellular Staining Flow Assay Kit . A: IMG-160A which recognizes only cleaved D4-GDI. B: which recognizes both full-length and cleaved.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of D4GD1-cv in formalin-fixed, paraffin-embedded human breast tumor tissue using an isotype control (top) and IMG-160A (bottom) at 5 μg/ml