MA1-41085

antibody from Invitrogen Antibodies

Targeting: ARHGDIB GDIA2, GDID4, Ly-GDI, RAP1GN1, RhoGDI2

Western blot

Immunoprecipitation

Immunohistochemistry

Antibody data

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Validation data

Product number: MA1-41085 - Provider product page
Provider: Invitrogen Antibodies
Product name: D4-GDI Monoclonal Antibody (97A1015)
Antibody type: Monoclonal
Antigen: Synthetic peptide
Description: Suggested positive control: anti-Fas treated Jurkat (10 ng/mL), antigen standard for ARHGDIB (transient overexpression lysate).
Reactivity: Human, Mouse
Host: Mouse
Isotype: IgG
Antibody clone number: 97A1015
Vial size: 100 µg
Concentration: 0.5 mg/mL
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

ARHGDIB protein structure - MA1-41085 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: HL60 cells were left untreated (green) or treated with camptothecin to induce apoptosis (red). Cells were analyzed by intracellular flow cytometry using either the a D4 GDI monoclonal antibody (Product # MA1-41085) or a D4 GDI monoclonal antibody (Product # MA1-41181) (0.5 µg antibody/test/1 million cells). A: a D4 GDI monoclonal antibody (Product # MA1-41085) which recognizes only cleaved D4-GDI. B: a D4 GDI monoclonal antibody (Product # MA1-41085) which recognizes both full-length and cleaved D4-GDI. The results in (A) show that the untreated cell population was primarily negative for cleaved D4-GDI (Product # MA1-41085) whereas the treated population was primarily positive. This is because D4-GDI is cleaved in cells undergoing apoptosis but is present as a full-length form in normal cells. Hence the cleavage specific D4-GDI antibody (Product # MA1-41085) does not recognize very many cells in the untreated population. There may be a small percentage of positive cells in untreated populations due to a basal level of apoptosis. In contrast both the untreated and treated cell populations were positive for D4-GDI using Product # MA1-41181 (as seen in B). This is because it recognizes both the full-length (cells not undergoing apoptosis) and cleaved (apoptotic cells) forms of D4-GDI.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Flow cytometry of D4-GDI in HL60 cells untreated (green) or treated with camptothecin to induce apoptosis (red). Samples were incubated in D4-GDI monoclonal antibody (Product # MA1-41085) using a dilution of 0.5 µg antibody/test/1 million cells.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Flow cytometry of D4-GDI in 10^6 untreated (left) and 3 hour staurosporine-treated (right) Jurkat cells. Samples were incubated in D4-GDI monoclonal (Product # MA1-41085) using a dilution of 0.5 µg. Green histogram represents isotype control; red represents anti-D4-GDI antibody. Image using the Alexa Fluor 647 format of this antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Flow cytometry of D4-GDI in 10^6 camptothecin-treated (red) and untreated (green) HL60 cells. Samples were incubated in D4-GDI monoclonal antibody (Product # MA1-41085) using a dilution of 0.5 µg. The shaded histogram represents cells without antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Flow cytometry of D4-GDI in Ramos cells. Samples were incubated in D4-GDI monoclonal antibody (Product # MA1-41085) using a dilution of 0.5 µg. Untreated (left) and staurosporine-treated (right).