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LearnMA1-41085
antibody from Invitrogen Antibodies
Targeting: ARHGDIB
GDIA2, GDID4, Ly-GDI, RAP1GN1, RhoGDI2
Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Flow cytometry [5]
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- Product number
- MA1-41085 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- D4-GDI Monoclonal Antibody (97A1015)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- Suggested positive control: anti-Fas treated Jurkat (10 ng/mL), antigen standard for ARHGDIB (transient overexpression lysate).
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 97A1015
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Association of D4-GDI expression with breast cancer progression.
Rivera Rosado LA, Rodriguez-Canales J, Zhang B
Cancer biomarkers : section A of Disease markers 2011-2012;10(3-4):163-73
Cancer biomarkers : section A of Disease markers 2011-2012;10(3-4):163-73
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of D4-GDI in Jurkat cells. Samples were incubated in D4-GDI monoclonal antibody (Product # MA1-41085 using a dilution of 0.1 µg/mL. Cells were treated with 2 µM staurosporine for different time periods. Specifically detects only the cleaved form of the protein.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of D4-GDI in HL60 cells. Samples were incubated in D4-GDI monoclonal antibody (Product # MA1-41085 using a dilution of 10 ng/mL. Harvested cells were lysed and resolved by SDS-PAGE and transferred onto membrane. D4-GDI/RhoGDI2 cleavage was detected.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of D4-GDI in formalin-fixed, paraffin-embedded human breast tumor tissue. Samples were incubated in D4-GDI monoclonal antibody (Product # MA1-41085). Isotype control (top) and this antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HL60 cells were left untreated (green) or treated with camptothecin to induce apoptosis (red). Cells were analyzed by intracellular flow cytometry using either the a D4 GDI monoclonal antibody (Product # MA1-41085) or a D4 GDI monoclonal antibody (Product # MA1-41181) (0.5 µg antibody/test/1 million cells). A: a D4 GDI monoclonal antibody (Product # MA1-41085) which recognizes only cleaved D4-GDI. B: a D4 GDI monoclonal antibody (Product # MA1-41085) which recognizes both full-length and cleaved D4-GDI. The results in (A) show that the untreated cell population was primarily negative for cleaved D4-GDI (Product # MA1-41085) whereas the treated population was primarily positive. This is because D4-GDI is cleaved in cells undergoing apoptosis but is present as a full-length form in normal cells. Hence the cleavage specific D4-GDI antibody (Product # MA1-41085) does not recognize very many cells in the untreated population. There may be a small percentage of positive cells in untreated populations due to a basal level of apoptosis. In contrast both the untreated and treated cell populations were positive for D4-GDI using Product # MA1-41181 (as seen in B). This is because it recognizes both the full-length (cells not undergoing apoptosis) and cleaved (apoptotic cells) forms of D4-GDI.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of D4-GDI in HL60 cells untreated (green) or treated with camptothecin to induce apoptosis (red). Samples were incubated in D4-GDI monoclonal antibody (Product # MA1-41085) using a dilution of 0.5 µg antibody/test/1 million cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of D4-GDI in 10^6 untreated (left) and 3 hour staurosporine-treated (right) Jurkat cells. Samples were incubated in D4-GDI monoclonal (Product # MA1-41085) using a dilution of 0.5 µg. Green histogram represents isotype control; red represents anti-D4-GDI antibody. Image using the Alexa Fluor 647 format of this antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of D4-GDI in 10^6 camptothecin-treated (red) and untreated (green) HL60 cells. Samples were incubated in D4-GDI monoclonal antibody (Product # MA1-41085) using a dilution of 0.5 µg. The shaded histogram represents cells without antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of D4-GDI in Ramos cells. Samples were incubated in D4-GDI monoclonal antibody (Product # MA1-41085) using a dilution of 0.5 µg. Untreated (left) and staurosporine-treated (right).
