A300-998A

antibody from Invitrogen Antibodies

Targeting: ARFGEF1 ARFGEP1, BIG1, DKFZP434L057, p200

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

A300-998A

Provider

Invitrogen Antibodies

Product name

BIG1/ARFGEF1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

4° C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human BIG1/ARFGEF1 by western blot. Samples: Whole cell lysate (25 µg) from RKO, LNCaP, HEK293T, Jurkat, and GaMG cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-BIG1/ARFGEF1 antibody (Product # A300-998A lot 2) used for WB at 0.1 µg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human BIG1/ARFGEF1 by western blot. Samples: Whole cell lysate (25 µg) from NIH 3T3, TCMK-1, and CT26 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-BIG1/ARFGEF1 antibody (Product # A300-998A lot 2) used for WB at 0.1 µg/mL. Detection: Chemiluminescence with an exposure time of 3 minutes.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human and mouse BIG1/ARFGEF1 by western blot (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 µg) and mouse NIH3T3 (M; 50 µg) cells. Antibody: Affinity purified rabbit anti-BIG1/ARFGEF1 antibody A300-998A used for WB at 0.1 µg/ml (A) and 1 µg/ml (B), and used for IP at 3 µg/mg lysate. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human BIG1/ARFGEF1 by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-BIG1/ARFGEF1 (Cat. No. A300-998A Lot1) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human BIG1/ARFGEF1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from RKO cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-BIG1/ARFGEF1 antibody (Product # A300-998A lot 2) used for IP at 6 µg per reaction. BIG1/ARFGEF1 was also immunoprecipitated by a previous lot of this antibody (A300-998A lot 1) and a second antibody against a different epitope of BIG1/ARFGEF1 (Antibody 2). For blotting immunoprecipitated BIG1/ARFGEF1 (Product # A300-998A) was used at 0.1 µg/mL. Detection: Chemiluminescence with an exposure time of 10 seconds.