NB100-55257

antibody from Novus Biologicals

Targeting: ARFGEF1 ARFGEP1, BIG1, DKFZP434L057, p200

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NB100-55257

Provider

Novus Biologicals

Proper citation

Novus Cat#NB100-55257, RRID:AB_2227456

Product name

Rabbit Polyclonal ARFGEF1 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 ul

Concentration

1.0 mg/ml

Storage

Store at 4C. Do not freeze.

References

Submitted references

Identification of GBF1 as a cellular factor required for hepatitis C virus RNA replication.

Goueslain L, Alsaleh K, Horellou P, Roingeard P, Descamps V, Duverlie G, Ciczora Y, Wychowski C, Dubuisson J, Rouillé Y
Journal of virology 2010 Jan;84(2):773-87

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: ARFGEF1 Antibody [NB100-55257] - Detection of Human and Mouse BIG1/ARFGEF1 on HeLa whole cell lysates using NB100-55257.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: ARFGEF1 Antibody [NB100-55257] - Section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-BIG1/ARFGEF1 used at a dilution of 1:250. Detection: DAB staining using Immunohistochemistry Accessory Kit. Epitope Retrieval Buffer-High pH was substituted for Epitope Retrieval Buffer-Reduced pH.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry: ARFGEF1 Antibody [NB100-55257] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-BIG1/ARFGEF1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB staining using Immunohistochemistry Accessory Kit.