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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [3]
- Immunoprecipitation [1]
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- Product number
- NBP2-43680 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal CCT5 Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified.
- Reactivity
- Human, Mouse, Hamster
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Investigating Chaperonin-Containing TCP-1 subunit 2 as an essential component of the chaperonin complex for tumorigenesis.
Showalter AE, Martini AC, Nierenberg D, Hosang K, Fahmi NA, Gopalan P, Khaled AS, Zhang W, Khaled AR
Scientific reports 2020 Jan 21;10(1):798
Scientific reports 2020 Jan 21;10(1):798
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CCT5 Antibody (639) [NBP2-43680] - Analysis of various whole cell extracts (30 ug) were separated by 7.5 % SDS-PAGE, and blotted with TCP1 epsilon antibody diluted by 1:1000.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CCT5 Antibody (639) [NBP2-43680] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with TCP1 epsilon antibody [639].
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CCT5 Antibody (639) [NBP2-43680] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CCT5 Antibody (639) diluted at 1:1000. HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: CCT5 Antibody (639) [NBP2-43680] - Analysis of TCP1 epsilon protein from 293T whole cell extracts using 5 ug of TCP1 epsilon antibody [639]. Western blot analysis was performed using TCP1 epsilon antibody [639]. EasyBlot anti-Rabbit IgG was used as a secondary reagent.
