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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- H00022948-M01 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00022948-M01, RRID:AB_425898
- Product name
- CCT5 monoclonal antibody (M01), clone 4E5-4B1
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full length recombinant CCT5.
- Antigen sequence
MASMGTLAFDEYGRPFLIIKDQDRKSRLMGLEALK
SHIMAAKAVANTMRTSLGPNGLDKMMVDKDGDVTV
TNDGATILSMMDVDHQIAKLMVELSKSQDDEIGDG
TTGVVVLAGALLEEAEQLLDRGIHPIRIADGYEQA
ARVAIEHLDKISDSVLVDIKDTEPLIQTAKTTLGS
KVVNSCHRQMAEIAVNAVLTVADMERRDVDFELIK
VEGKVGGRLEDTKLIKGVIVDKDFSHPQMPKKVED
AKIAILTCPFEPPKPKTKHKLDVTSVEDYKALQKY
EKEKFEEMIQQIKETGANLAICQWGFDDEANHLLL
QNNLPAVRWVGGPEIELIAIATGGRIVPRFSELTA
EKLGFAGLVQEISFGTTKDKMLVIEQCKNSRAVTI
FIRGGNKMIIEEAKRSLHDALCVIRNLIRDNRVVY
GGGAAEISCALAVSQEADKCPTLEQYAMRAFADAL
EVIPMALSENSGMNPIQTMTEVRARQVKEMNPALG
IDCLHKGTNDMKQQHVIETLIGKKQQISLATQMVR
MILKIDDIRKPGESEE- Isotype
- IgG
- Antibody clone number
- 4E5-4B1
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Submitted references A proteomic approach to study parathyroid glands.
Evaluation of formalin-fixed paraffin-embedded tissues in the proteomic analysis of parathyroid glands.
Chaperonin TRiC/CCT participates in replication of hepatitis C virus genome via interaction with the viral NS5B protein.
Giusti L, Cetani F, Ciregia F, Da Valle Y, Donadio E, Giannaccini G, Banti C, Pardi E, Saponaro F, Basolo F, Berti P, Miccoli P, Pinchera A, Marcocci C, Lucacchini A
Molecular bioSystems 2011 Mar;7(3):687-99
Molecular bioSystems 2011 Mar;7(3):687-99
Evaluation of formalin-fixed paraffin-embedded tissues in the proteomic analysis of parathyroid glands.
Donadio E, Giusti L, Cetani F, Da Valle Y, Ciregia F, Giannaccini G, Pardi E, Saponaro F, Torregrossa L, Basolo F, Marcocci C, Lucacchini A
Proteome science 2011 Jun 8;9(1):29
Proteome science 2011 Jun 8;9(1):29
Chaperonin TRiC/CCT participates in replication of hepatitis C virus genome via interaction with the viral NS5B protein.
Inoue Y, Aizaki H, Hara H, Matsuda M, Ando T, Shimoji T, Murakami K, Masaki T, Shoji I, Homma S, Matsuura Y, Miyamura T, Wakita T, Suzuki T
Virology 2011 Feb 5;410(1):38-47
Virology 2011 Feb 5;410(1):38-47
No comments: Submit comment
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- CCT5 monoclonal antibody (M01), clone 4E5-4B1 Western Blot analysis of CCT5 expression in Hela ( Cat # L013V1 ).
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged CCT5 is approximately 0.3ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence of monoclonal antibody to CCT5 on HeLa cell. [antibody concentration 10 ug/ml]
- Validation comment
- Immunofluorescence
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoperoxidase of monoclonal antibody to CCT5 on formalin-fixed paraffin-embedded human lymphoma tissue. [antibody concentration 1 ug/ml]
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Protocol
- Protocol
