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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PA5-22093 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TCP-1 epsilon Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, Molt-4, Mouse brain. Predicted reactivity: Mouse (94%), Rat (94%), Zebrafish (83%), Xenopus laevis (86%), Pig (96%), Chicken (88%), Rhesus Monkey (100%), Chimpanzee (100%), Bovine (95%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of TCP-1 epsilon using Whole cell extracts (30 µg). Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a TCP-1 epsilon polyclonal antibody (Product # PA5-22093) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using TCP-1 epsilon Polyclonal Antibody (Product # PA5-22093). Sample (30 µg of whole cell lysate). Lane A: Molt-4 . 7.5% SDS PAGE. TCP-1 epsilon Polyclonal Antibody (Product # PA5-22093) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using TCP-1 epsilon Polyclonal Antibody (Product # PA5-22093). Sample (50 µg of whole cell lysate). Lane A: mouse brain . 7.5% SDS PAGE. TCP-1 epsilon Polyclonal Antibody (Product # PA5-22093) diluted at 1:10,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of TCP-1 epsilon in paraformaldehyde-fixed HeLa cells using a TCP-1 epsilon polyclonal antibody (Product # PA5-22093) at a 1:200 dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using TCP-1 epsilon antibody (Product # PA5-22093) at 1:200 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of TCP-1 epsilon was performed in paraffin-embedded human breast carcinoma tissue using TCP-1 epsilon Polyclonal Antibody (Product # PA5-22093) at a dilution of 1:500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of TCP1 epsilon was performed in 293T whole cell extracts using 5 µg of TCP-1 epsilon Polyclonal Antibody (Product # PA5-22093). Samples were transferred to a membrane and probed with TCP-1 epsilon Polyclonal Antibody as a primary antibody and an HRP-conjugated anti-Rabbit IgG was used as a secondary antibody.
