H00000701-M03

antibody from Abnova Corporation

Targeting: BUB1B Bub1A, BUBR1, MAD3L, SSK1

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

H00000701-M03

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00000701-M03, RRID:AB_518684

Product name

BUB1B monoclonal antibody (M03), clone 3F2

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a partial recombinant BUB1B.

Antigen sequence

MAAVKKEGGALSEAMSLEGDEWELSKENVQPLRQG
RIMSTLQGALAQESACNNTLQQQKRAFEYEIRFYT
GNDPLDVWDRYISWTEQNYPQGGKESNMSTLLERA
VEALQGEKRYYSDPRFLNLWLKLGR


Isotype

IgG

Antibody clone number

3F2

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

BUB1B monoclonal antibody (M03), clone 3F2 Western Blot analysis of BUB1B expression in Hela S3 NE ( Cat # L013V3 ).

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged BUB1B is approximately 0.03ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunoperoxidase of monoclonal antibody to BUB1B on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 1 ug/ml]

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Protocol

Protocol