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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- MAB8964 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#MAB8964, RRID:AB_10716005
- Product name
- BUB1B monoclonal antibody, clone P10.8G1.G11.H12
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against BUB1B.
- Isotype
- IgG
- Antibody clone number
- P10.8G1.G11.H12
- Storage
- Store at -20°C.Aliquot to avoid repeated freezing and thawing.
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Supportive validation
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- Abnova Corporation (provider)
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- Experimental details
- Western blot analysis in K562 and HeLa cells were separated according to their position in the cell cycle by centrifugal elutriation. BUB1B was immunoprecipitated from equal amouts of protein from each fraction, and then tested for autokinase activity (top) or probed with BUB1B antibodies to compare its cell cycle expression profile (middle and bottom). with BUB1B monoclonal antibody, clone P10.8G1.G11.H12 (Cat # MAB8964).
