TA500679

antibody from OriGene

Targeting: BUB1B Bub1A, BUBR1, MAD3L, SSK1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA500679

Provider

OriGene

Proper citation

OriGene Cat#TA500679, RRID:AB_11129205

Product name

BuB1B (BubR1) mouse monoclonal antibody, clone OTI6E3 (formerly 6E3)

Antibody type

Monoclonal

Description

BuB1B (BubR1) mouse monoclonal antibody, clone OTI6E3 (formerly 6E3)

Reactivity

Canine

Host

Mouse

Conjugate

Unconjugated

Epitope

BUB1B

Isotype

IgG

Antibody clone number

OTI6E3

Vial size

100 µl

Concentration

1.00mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BUB1B (RC205844, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BUB1B.

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-BUB1B monoclonal antibody.

Validation comment

WB

Immunocytochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Anti-BUB1B mouse monoclonal antibody (TA500679) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BUB1B(RC205844).

Validation comment

IF

Flow cytometry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Flow cytometric Analysis of Jurkat cells, using anti-BUB1B antibody(TA500679),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).

Validation comment

FC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells transfected with either RC205844 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-BUB1B antibody(TA500679), and then analyzed by flow cytometry.

Validation comment

FC