LS-C677327

antibody from LSBio

Targeting: BUB1B Bub1A, BUBR1, MAD3L, SSK1

ELISA (Recommended by provider)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

LS-C677327

Provider

LSBio

Product name

BUB1B / BubR1 Antibody (aa230-394) LS-C677327

Antibody type

Polyclonal

Description

Protein G purified

Reactivity

Human

Host

Rabbit

Isotype

IgG

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Immunoprecipitation

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunoprecipitating BUB1B in JK whole cell lysate Lane 1: Rabbit control IgG (1µg) instead of product in Jurkat whole cell lysate.For western blotting,a HRP-conjugated Protein G antibody was used as the Secondary antibody (1/2000) Lane 2: product (6µg) + JK whole cell lysate (500µg) Lane 3: JK whole cell lysate (10µg)

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunoprecipitating BUB1B in JK whole cell lysate Lane 1: Rabbit control IgG (1µg) instead of product in Jurkat whole cell lysate.For western blotting,a HRP-conjugated Protein G antibody was used as the Secondary antibody (1/2000) Lane 2: product (6µg) + JK whole cell lysate (500µg) Lane 3: JK whole cell lysate (10µg)

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunoprecipitating BUB1B in JK whole cell lysate Lane 1: Rabbit control IgG (1µg) instead of product in Jurkat whole cell lysate.For western blotting,a HRP-conjugated Protein G antibody was used as the Secondary antibody (1/2000) Lane 2: product (6µg) + JK whole cell lysate (500µg) Lane 3: JK whole cell lysate (10µg)