ABIN251432

antibody from antibodies-online

Targeting: BUB1B Bub1A, BUBR1, MAD3L, SSK1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

ABIN251432

Provider

antibodies-online

Product name

anti-Budding Uninhibited By Benzimidazoles 1 Homolog beta (Yeast) (BUB1B) (AA 1-350), (N-Term) antibody

Antibody type

Monoclonal

Description

Protein G purified

Reactivity

Human, Mouse

Host

Mouse

Epitope

N-Term, AA 1-350

Isotype

IgG

Antibody clone number

8G1

Vial size

0.025 mL

Storage

Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.

Handling

Avoid freeze-thaw cycles

Western blot

Supportive validation

Submitted by

antibodies-online (provider)

Main image

Experimental details

WB

Supportive validation

Submitted by

antibodies-online (provider)

Main image

Experimental details

WB

Immunocytochemistry

Supportive validation

Submitted by

antibodies-online (provider)

Main image

Experimental details

Immunofluorescence of an asynchronous cycling population of human cells (U2OS) with ABIN151818. No signal is detected from interphase cells, whereas cells undergoing mitosis accumulate BubR1 at the kinetochores. Image reveals kinetochores at prometaphase. Image courtesy of Luke Hughes-Davies and Rhiannon Jade, Gurdon Institute, Cambridge, UK

Flow cytometry

Supportive validation

Submitted by

antibodies-online (provider)

Main image

Experimental details

FACS

Supportive validation

Submitted by

antibodies-online (provider)

Main image

Experimental details

FACS