Explore
Validate
Learn
Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Western blot [3]
Submit
Validation data
Reference
Comment
Report error
- Product number
- NB100-579 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-579, RRID:AB_10001919
- Product name
- Rabbit Polyclonal BubR1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references The Ndc80 internal loop is required for recruitment of the Ska complex to establish end-on microtubule attachment to kinetochores.
Inhibitory phosphorylation of cyclin-dependent kinase 1 as a compensatory mechanism for mitosis exit.
Orderly inactivation of the key checkpoint protein mitotic arrest deficient 2 (MAD2) during mitotic progression.
Inhibitory factors associated with anaphase-promoting complex/cylosome in mitotic checkpoint.
Checkpoint kinase 1 (Chk1) is required for mitotic progression through negative regulation of polo-like kinase 1 (Plk1).
Zhang G, Kelstrup CD, Hu XW, Kaas Hansen MJ, Singleton MR, Olsen JV, Nilsson J
Journal of cell science 2012 Jul 1;125(Pt 13):3243-53
Journal of cell science 2012 Jul 1;125(Pt 13):3243-53
Inhibitory phosphorylation of cyclin-dependent kinase 1 as a compensatory mechanism for mitosis exit.
Chow JP, Poon RY, Ma HT
Molecular and cellular biology 2011 Apr;31(7):1478-91
Molecular and cellular biology 2011 Apr;31(7):1478-91
Orderly inactivation of the key checkpoint protein mitotic arrest deficient 2 (MAD2) during mitotic progression.
Ma HT, Poon RY
The Journal of biological chemistry 2011 Apr 15;286(15):13052-9
The Journal of biological chemistry 2011 Apr 15;286(15):13052-9
Inhibitory factors associated with anaphase-promoting complex/cylosome in mitotic checkpoint.
Braunstein I, Miniowitz S, Moshe Y, Hershko A
Proceedings of the National Academy of Sciences of the United States of America 2007 Mar 20;104(12):4870-5
Proceedings of the National Academy of Sciences of the United States of America 2007 Mar 20;104(12):4870-5
Checkpoint kinase 1 (Chk1) is required for mitotic progression through negative regulation of polo-like kinase 1 (Plk1).
Tang J, Erikson RL, Liu X
Proceedings of the National Academy of Sciences of the United States of America 2006 Aug 8;103(32):11964-9
Proceedings of the National Academy of Sciences of the United States of America 2006 Aug 8;103(32):11964-9
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: BubR1 Antibody [NB100-579] - Detection of Mouse and Rat BubR1 by Western Blot. Samples: Whole cell lysate (50 ug) from NIH3T3, TCMK-1, 4T1, CT26.WT, and rat C6 cells. Antibodies: Affinity purified rabbit anti-BubR1 antibody NB100-579 used for WB at 0.2 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: BubR1 Antibody [NB100-579] - Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-BubR1 antibody NB100-579 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: BubR1 Antibody [NB100-579] - Detection of Human BubR1 on HeLa whole cell lysate using NB100-579. BubR1 was also immunoprecipitated using another affinity purified rabbit anti-BubR1 antibody to a different epitope.
