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LearnMAB1429-100
antibody from R&D Systems
Targeting: CCR8
CDw198, CKR-L1, CMKBR8, CMKBRL2, CY6, GPR-CY6, TER1
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Flow cytometry [1]
- Blocking/Neutralizing [1]
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- Product number
- MAB1429-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human CCR8 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human CCR8. Neutralizes the bioactivity of human CCR8 and specifically stains human CCR8-transfected cell lines but not with the parent cell lines.
- Reactivity
- Human
- Host
- Rat
- Conjugate
- Unconjugated
- Antigen sequence
P51685- Isotype
- IgG
- Antibody clone number
- 191704
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Distribution, persistence, and efficacy of adoptively transferred central and effector memory-derived autologous simian immunodeficiency virus-specific CD8+ T cell clones in rhesus macaques during acute infection.
Trafficking, persistence, and activation state of adoptively transferred allogeneic and autologous Simian Immunodeficiency Virus-specific CD8(+) T cell clones during acute and chronic infection of rhesus macaques.
Minang JT, Trivett MT, Bolton DL, Trubey CM, Estes JD, Li Y, Smedley J, Pung R, Rosati M, Jalah R, Pavlakis GN, Felber BK, Piatak M Jr, Roederer M, Lifson JD, Ott DE, Ohlen C
Journal of immunology (Baltimore, Md. : 1950) 2010 Jan 1;184(1):315-26
Journal of immunology (Baltimore, Md. : 1950) 2010 Jan 1;184(1):315-26
Trafficking, persistence, and activation state of adoptively transferred allogeneic and autologous Simian Immunodeficiency Virus-specific CD8(+) T cell clones during acute and chronic infection of rhesus macaques.
Bolton DL, Minang JT, Trivett MT, Song K, Tuscher JJ, Li Y, Piatak M Jr, O'Connor D, Lifson JD, Roederer M, Ohlen C
Journal of immunology (Baltimore, Md. : 1950) 2010 Jan 1;184(1):303-14
Journal of immunology (Baltimore, Md. : 1950) 2010 Jan 1;184(1):303-14
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of CCR8 in Human Blood by Flow Cytometry. Human peripheral blood cells were stained with (A) Rat Anti-Human CCR8 Monoclonal Antibody (Catalog # MAB1429) or (B) isotype control antibody (Catalog # MAB0061) followed by anti-Rat IgG APC-conjugated Secondary Antibody (Catalog # F0113) and Mouse anti-Human CD14 PE-conjugated Monoclonal Antibody (Catalog # FAB3832P). View our protocol for Staining Membrane-associated Proteins.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Chemotaxis Induced by CCL1/I-309 and Neutralization by Human CCR8 Antibody. Recombinant Human CCL1/I-309 (Catalog # 272-I) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR8 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL1/I-309 (20 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Human CCR8 Monoclonal Antibody (Catalog # MAB1429). The ND50 is typically 0.01-0.05 µg/mL.
