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LearnAF5997
antibody from R&D Systems
Targeting: CCR8
CDw198, CKR-L1, CMKBR8, CMKBRL2, CY6, GPR-CY6, TER1
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
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- Product number
- AF5997 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse/Rat CCR8 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects mouse and rat CCR8 in direct ELISAs and Western blots. In direct ELISAs, less than 2% cross-reactivity with recombinant mouse (rm) CCR1, rmCCR4, and rmCCR5 is observed.
- Reactivity
- Mouse, Rat
- Host
- Sheep
- Conjugate
- Unconjugated
- Antigen sequence
P56484- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Mouse and Rat CCR8 by Western Blot. Western blot shows lysates of mouse thymus tissue and rat thymus tissue. PVDF Membrane was probed with 0.5 µg/mL of Sheep Anti-Mouse/Rat CCR8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5997) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CCR8 at approximately 41-43 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
