Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- PB9254 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-Integrin alpha 5/ITGA5 Antibody Picoband™
- Antibody type
- Polyclonal
- Description
- Polyclonal antibody for CD49e/ITGA5 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: Flow Cytometry. Reactive species: Human. CD49e/ITGA5 information: Molecular Weight: 114536 MW; Subcellular Localization: Membrane; Single-pass type I membrane protein.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Vial size
- 100μg/vial
- Concentration
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
- Storage
- At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
- Handling
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Submitted references Avian Hepatitis E Virus ORF2 Protein Interacts with Rap1b to Induce Cytoskeleton Rearrangement That Facilitates Virus Internalization.
Revealing the pathogenic changes of PAH based on multiomics characteristics.
A small physiological electric field mediated responses of extravillous trophoblasts derived from HTR8/SVneo cells: involvement of activation of focal adhesion kinase signaling.
Zhang B, Fan M, Fan J, Luo Y, Wang J, Wang Y, Liu B, Sun Y, Zhao Q, Hiscox JA, Nan Y, Zhou EM
Microbiology spectrum 2022 Feb 23;10(1):e0226521
Microbiology spectrum 2022 Feb 23;10(1):e0226521
Revealing the pathogenic changes of PAH based on multiomics characteristics.
Zhang L, Chen S, Zeng X, Lin D, Li Y, Gui L, Lin MJ
Journal of translational medicine 2019 Jul 22;17(1):231
Journal of translational medicine 2019 Jul 22;17(1):231
A small physiological electric field mediated responses of extravillous trophoblasts derived from HTR8/SVneo cells: involvement of activation of focal adhesion kinase signaling.
Zhang J, Ren R, Luo X, Fan P, Liu X, Liang S, Ma L, Yu P, Bai H
PloS one 2014;9(3):e92252
PloS one 2014;9(3):e92252
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Supportive validation
- Submitted by
- Boster Biological Technology (provider)
- Main image
- Experimental details
- Western blot analysis of Integrin alpha 5 using anti-Integrin alpha 5 antibody (PB9254). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Recombinant Human Integrin alpha 5 Protein 0.5ng After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Integrin alpha 5 antigen affinity purified polyclonal antibody (Catalog # PB9254) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Integrin alpha 5 at approximately 39KD. The expected band size for Integrin alpha 5 is at 39KD.
- Additional image