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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [6]
- Comments [0]
- Validations
- Flow cytometry [1]
- Other assay [1]
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- Product number
- 53-0496-41 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD49e (Integrin alpha 5) Monoclonal Antibody (eBioSAM-1 (SAM-1, SAM1)), Alexa Fluor™ 488, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- HWA4C4 reacts specifically with the isopeptide linkage region of lysine-63-linked polyubiquitin. It does not react with other forms of ubiquitin including ubiquitin monomer and lysine-48-linked chains.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Green dye
- Isotype
- IgG
- Antibody clone number
- eBioSAM-1 (SAM-1, SAM1)
- Vial size
- 25 Tests
- Concentration
- 5 µL/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references Human SND2 mediates ER targeting of GPI-anchored proteins with low hydrophobic GPI attachment signals.
Comprehensive Cell Surface Antigen Analysis Identifies Transferrin Receptor Protein-1 (CD71) as a Negative Selection Marker for Human Neuronal Cells.
Porcine mononuclear cells adhere to human fibronectin independently of very late antigen-5: implications for donor-specific tolerance induction in xenotransplantation.
Monocyte-derived dendritic cells have a phenotype comparable to that of dermal dendritic cells and display ultrastructural granules distinct from Birbeck granules.
Signal transduction through the beta1 integrin family surface adhesion molecules VLA-4 and VLA-5 of human B-cell precursors activates CD19 receptor-associated protein-tyrosine kinases.
An alpha 5 beta 1-like integrin receptor mediates the binding of less pathogenic Candida species to fibronectin.
Yang J, Hirata T, Liu YS, Guo XY, Gao XD, Kinoshita T, Fujita M
FEBS letters 2021 Jun;595(11):1542-1558
FEBS letters 2021 Jun;595(11):1542-1558
Comprehensive Cell Surface Antigen Analysis Identifies Transferrin Receptor Protein-1 (CD71) as a Negative Selection Marker for Human Neuronal Cells.
Menon V, Thomas R, Elgueta C, Horl M, Osborn T, Hallett PJ, Bartos M, Isacson O, Pruszak J
Stem cells (Dayton, Ohio) 2019 Oct;37(10):1293-1306
Stem cells (Dayton, Ohio) 2019 Oct;37(10):1293-1306
Porcine mononuclear cells adhere to human fibronectin independently of very late antigen-5: implications for donor-specific tolerance induction in xenotransplantation.
Theodore PR, Simon AR, Warrens AN, Sackstein R, Sykes M
Xenotransplantation 2002 Jul;9(4):277-89
Xenotransplantation 2002 Jul;9(4):277-89
Monocyte-derived dendritic cells have a phenotype comparable to that of dermal dendritic cells and display ultrastructural granules distinct from Birbeck granules.
Grassi F, Dezutter-Dambuyant C, McIlroy D, Jacquet C, Yoneda K, Imamura S, Boumsell L, Schmitt D, Autran B, Debré P, Hosmalin A
Journal of leukocyte biology 1998 Oct;64(4):484-93
Journal of leukocyte biology 1998 Oct;64(4):484-93
Signal transduction through the beta1 integrin family surface adhesion molecules VLA-4 and VLA-5 of human B-cell precursors activates CD19 receptor-associated protein-tyrosine kinases.
Xiao J, Messinger Y, Jin J, Myers DE, Bolen JB, Uckun FM
The Journal of biological chemistry 1996 Mar 29;271(13):7659-64
The Journal of biological chemistry 1996 Mar 29;271(13):7659-64
An alpha 5 beta 1-like integrin receptor mediates the binding of less pathogenic Candida species to fibronectin.
Santoni G, Birarelli P, Hong LJ, Gamero A, Djeu JY, Piccoli M
Journal of medical microbiology 1995 Nov;43(5):360-7
Journal of medical microbiology 1995 Nov;43(5):360-7
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of normal human peripheral blood cells with Anti-Human CD3 APC (Product # 17-0038-42) and Mouse IgG1 kappa Isotype Control Alexa Fluor® 488 (Product # 53-4714-42) (left) or Anti-Human CD49e (Integrin alpha 5) Alexa Fluor® 488 (right). Cells in the lymphocyte gate were used for analysis.
- Conjugate
- Green dye
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 1 Fig. Knockout of hSND2 causes a reduction in GPI-AP expression. (A) Schematic representation of the hSND2 protein. The regions corresponding to the gene deletion using CRISPR/Cas9 systems are shown as black arrows. Two sgRNA sequences were used for knocking out hSND2. TM, transmembrane domain. (B) Confirmation of hSND2 gene KO. Genomic DNA was extracted from wild-type and hSND2-KO No. 5 clonal cell lines, and a DNA fragment was amplified around the KO region. A decreased DNA band was detected in the hSND2-KO cells compared with the wild-type cells. The expected size of the DNA fragment for hSND2 is 206 bp in wild-type cells and 135 bp in KO cells. The 71-bp sequence was deleted in the hSND2-KO No. 5 cell line. Capital bold letters, the target sequences; underlined letters, the PAM sequence; dotted lines, the deletion sequence. WT, wild-type cells; KO, knockout cells. (C) Flow cytometric analysis of the CD59, DAF, CD109, BSG, and CD49e proteins in HEK293 and hSND2-KO cells. The cells were stained with appropriate antibodies. Black solid lines, each protein's staining in HEK293 cells; gray shaded areas, each protein's staining in hSND2-KO cells; dashed black lines, HEK293 cells only stained with second antibody (background). (D) Analysis and graphical representation of C. Quantitative data of the mean fluorescence intensity of three independent experiments are shown as the means +- SD. The value in HEK293 cells was set as 1, and the relative values in hSND2-KO cells are shown
- Conjugate
- Green dye
