AMAb91448

antibody from Atlas Antibodies

Targeting: ITGA5 CD49e, FNRA

Immunohistochemistry (Enhanced validation)

Antibody Data

Product number

AMAb91448

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#AMAb91448, RRID:AB_2732102

Product name

Anti-ITGA5

Antibody type

Monoclonal

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Antigen sequence

DQPQKEEDLGPAVHHVYELINQGPSSISQGVLELS
CPQALEGQQLLYVTRVTGLNCTTNHPINPKGLELD
PEGSLHHQQKREAPSRSSASSGPQILKCPEAECFR
LRCELGPLHQQESQSLQLHFRVWA


Epitope

Binds to an epitope located within the peptide sequence LRCELGPLHQQESQS as determined by overlapping synthetic peptides.

Isotype

IgG

Antibody clone number

CL6945

Vial size

100 µl

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Immunohistochemistry

Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Orthogonal validation

Main image

Experimental details

Immunohistochemistry analysis in human placenta and testis tissues using AMAb91448 antibody. Corresponding ITGA5 RNA-seq data are presented for the same tissues.

Sample type

HUMAN

Orthogonal method

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

Show more

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human placenta shows strong apical membrane positivity in trophoblastic cells.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human prostate shows moderate to strong membranous positivity in smooth muscle cells.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human liver shows moderate positivity in liver sinusoids.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human testis shows no positivity in cells in seminiferous ducts as expected.