17-2999-41
antibody from Invitrogen Antibodies
Targeting: CLEC4M
CD209L, CD299, DC-SIGN2, DC-SIGNR, DCSIGNR, HP10347, LSIGN
Antibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Flow cytometry [1]
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Validation data
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- Product number
- 17-2999-41 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-CD299 (DC-SIGN/L) Monoclonal Antibody (16E7), APC, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The 16E7 monoclonal antibody recognizes human CD299, which is also known as DC-SIGNR or L-SIGN. A homolog of DC-SIGN, CD299 is an oligomeric type II transmembrane protein expressed on dendritic cells, macrophages in the lung, and endothelial cells in the liver. This C-type lectin binds ICAM-3 to mediate the interaction between dendritic cells and T cells. CD299 also binds the gp120 protein of HIV and the E2 envelope protein of HCV, thereby playing a role in viral infection. Applications Reported: This 16E7 antibody has been reported for use in flow cytometric analysis. Applications Tested: This 16E7 antibody has been pre-titrated and tested by flow cytometric analysis of normal human monocyte-derived dendritic cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 16E7
- Vial size
- 25 Tests
- Concentration
- 5 µL/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references DC-SIGN and DC-SIGNR bind ebola glycoproteins and enhance infection of macrophages and endothelial cells.
Hepatitis C virus glycoproteins interact with DC-SIGN and DC-SIGNR.
DC-SIGNR, a DC-SIGN homologue expressed in endothelial cells, binds to human and simian immunodeficiency viruses and activates infection in trans.
A novel mechanism of carbohydrate recognition by the C-type lectins DC-SIGN and DC-SIGNR. Subunit organization and binding to multivalent ligands.
Simmons G, Reeves JD, Grogan CC, Vandenberghe LH, Baribaud F, Whitbeck JC, Burke E, Buchmeier MJ, Soilleux EJ, Riley JL, Doms RW, Bates P, Pöhlmann S
Virology 2003 Jan 5;305(1):115-23
Virology 2003 Jan 5;305(1):115-23
Hepatitis C virus glycoproteins interact with DC-SIGN and DC-SIGNR.
Pöhlmann S, Zhang J, Baribaud F, Chen Z, Leslie GJ, Lin G, Granelli-Piperno A, Doms RW, Rice CM, McKeating JA
Journal of virology 2003 Apr;77(7):4070-80
Journal of virology 2003 Apr;77(7):4070-80
DC-SIGNR, a DC-SIGN homologue expressed in endothelial cells, binds to human and simian immunodeficiency viruses and activates infection in trans.
Pöhlmann S, Soilleux EJ, Baribaud F, Leslie GJ, Morris LS, Trowsdale J, Lee B, Coleman N, Doms RW
Proceedings of the National Academy of Sciences of the United States of America 2001 Feb 27;98(5):2670-5
Proceedings of the National Academy of Sciences of the United States of America 2001 Feb 27;98(5):2670-5
A novel mechanism of carbohydrate recognition by the C-type lectins DC-SIGN and DC-SIGNR. Subunit organization and binding to multivalent ligands.
Mitchell DA, Fadden AJ, Drickamer K
The Journal of biological chemistry 2001 Aug 3;276(31):28939-45
The Journal of biological chemistry 2001 Aug 3;276(31):28939-45
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Supportive validation
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of normal human monocyte-derived dendritic cells with Mouse IgG2a K Isotype Control APC (Product # 17-4724-81) (blue histogram) or Anti-Human CD299 (DC-SIGN/L) APC (purple histogram). Total viable cells were used for analysis.