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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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Validation data
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- Product number
- 209-403-B94 - Provider product page
- Provider
- Rockland Immunochemicals, Inc.
- Proper citation
- Rockland Cat#209-403-B94, RRID:AB_10704797
- Product name
- Anti-Human IL-7 (RABBIT) Antibody Peroxidase Conjugated - 209-403-B94
- Antibody type
- Polyclonal
- Vial size
- 100 µl
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Supportive validation
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- Rockland Immunochemicals, Inc. (provider)
- Main image
- Experimental details
- Rockland's anti-Human IL-7 antibody shows detection of a band ~17 kDa in size corresponding to recombinant human IL-7. The identity of the faint higher molecular weight band may represent a homodimer. Molecular weight markers are also shown (left). After transfer, the membrane was blocked overnight with 3% BSA in TBS followed by reaction with primary antibody at a 1:1,000 dilution. Detection occurred using peroxidase conjugated anti-Rabbit IgG (p/n 611-103-122) secondary antibody diluted 1:40,000 in blocking buffer (p/n MB-070) for 30 min at RT followed by reaction with FemtoMax chemiluminescent substrate. Image was captured using VersaDoc MP 4000 imaging system (Bio-Rad).
- Validation comment
- Western Blot
