600-401-446

antibody from Rockland Immunochemicals, Inc.

Targeting: PLK1 PLK

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

600-401-446

Provider

Rockland Immunochemicals, Inc.

Proper citation

Rockland Cat#600-401-446, RRID:AB_2167429

Product name

Anti-Polo-like Kinase (Plk1) pT210 (RABBIT) Antibody - 600-401-446

Antibody type

Polyclonal

Vial size

100 µl

Western blot

Supportive validation

Submitted by

Rockland Immunochemicals, Inc. (provider)

Main image

Experimental details

Western blot analysis is shown to detect endogenous and recombinant protein present in HeLa cell lysates transfected with various plk-1 mutation constructs. Blots were reacted with anti-Plk-1 pT210 (panel A) and pan reactive anti-Plk-1 (panel B). Transfected cells were treated with 1 uM nocodazole followed by cell collection, lysate preparation, SDS-PAGE and western blotting. Using a 1:1000 dilution, anti-Plk-1 pT210 detects a single band corresponding to endogenous plk-1, but does not detect recombinant forms of the protein presumably because of a lack of phosphorylation in these mutants. Personal communication Hai Jiang, Northwestern Univ.

Validation comment

Western Blot