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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [7]
- Immunohistochemistry [2]
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- Product number
- NBP2-15975 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal COX4 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: COX IV isoform 1 Antibody [NBP2-15975] - Sample (30 ug of whole cell lysate) A: Hep G2 12% SDS PAGE gel, diluted at 1:1000.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: COX4 Antibody [NBP2-15975] - Sample (50 ug of whole cell lysate) A: rat muscle, 12% SDS PAGE gel, diluted at 1:500.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: COX4 Antibody [NBP2-15975] - Sample (50 ug of whole cell lysate) A: Mouse Muscle, 12% SDS PAGE gel, diluted at 1:500.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: COX4 Antibody [NBP2-15975] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with COX4 antibody. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: COX4 Antibody [NBP2-15975] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with COX4 antibody diluted at 1:1000.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: COX4 Antibody [NBP2-15975] - Various tissue extracts (50 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with COX4 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: COX4 Antibody [NBP2-15975] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with COX4 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: COX4 Antibody [NBP2-15975] - HS578T xenograft, using COX4 antibody at 1:250 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: COX4 Antibody [NBP2-15975] - COX4 antibody detects COX4 protein at mitochondria by immunohistochemical analysis. Sample: Paraffin-embedded human endometrial carcinoma. COX4 stained by COX4 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
