LS-B10751

antibody from LSBio

Targeting: COX4I1 COX4, COX4-1

Western blot (Enhanced validation)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Enhanced validation)

Antibody Data

Product number

LS-B10751

Provider

LSBio

Product name

IHC-plus™ COXIV / COX4 Antibody (aa1-169) LS-B10751

Antibody type

Polyclonal

Description

Immunoaffinity purified

Reactivity

Human

Host

Rabbit

Isotype

IgG

Storage

Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Western blot

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Sample (30 ug of whole cell lysate). A: A431, B: MOLT4. 12% SDS PAGE. COXIV / COX4 antibody diluted at 1:2000

Immunocytochemistry

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa using COX4 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with alpha-tubulin antibody (Red) at 1:2000.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa using COX4 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with alpha-tubulin antibody (Red) at 1:2000.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa using COX4 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with alpha-tubulin antibody (Red) at 1:2000.

Immunohistochemistry

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Anti-COXIV / COX4 antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 7.5 ug/ml.

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Anti-COXIV / COX4 antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 7.5 ug/ml.