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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- ARG54003 - Provider product page
- Provider
- Arigo
- Product name
- anti-COX IV antibody
- Antibody type
- Monoclonal
- Antigen
- A synthetic peptide corresponding to carboxyl terminal residues of human COX IV
- Description
- Affinity purified
- Reactivity
- Human, Mouse, Rat, Goat, Hamster, Simian
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 4D11-B3-E8
- Vial size
- 100 µl
- Concentration
- 1mg/ml
- Storage
- Aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. The antibody solution should be gently mixed before use.
- Handling
- The antibody solution should be gently mixed before use.
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Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Western Blot: COX IV in Mouse skeletal muscel and Jurkat lysates stained with COX IV antibody [4D11-B3-E8] (ARG54003) (diluted in 1: 1000). Predicted band size: 17KDa. Observed band size: 17KDa.
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Western Blot: COX IV in Goat muscle, CHO-k1, COS7, 3T3, HeLa, C6 and K562 cell lysates stained with COX IV antibody [4D11-B3-E8] (ARG54003) (diluted in 1: 5000). Predicted band size: 17KDa. Observed band size: 17KDa.
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunofluorescence: HeLa cells stained with COX IV antibody [4D11-B3-E8] (ARG54003) (diluted in 1:150).
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunoprecipitation: HeLa cell lysates immunoprecipitated and stained by COX IV antibody [4D11-B3-E8] (ARG54003).
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunohistochemistry: paraffin-embedded human colorectal carcinoma with COX IV antibody [4D11-B3-E8] (ARG54003) (4D11-B3-E8, 1:50) , showing cytoplasm localization. A high pressure mediated antigen retrieval step was performed in citrate buffer (pH6. 0).
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Flow Cytometry: K562 cells stained with COX IV antibody [4D11-B3-E8] (ARG54003) (red, 1/100), followed by FITC-conjugated goat mouse IgG. Blue line histogram represents the isotype control, normal mouse IgG.
