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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- TA346921 - Provider product page
- Provider
- OriGene
- Product name
- Mouse Monoclonal COX IV Antibody
- Antibody type
- Monoclonal
- Description
- Mouse Monoclonal COX IV Antibody
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- COX4I1
- Isotype
- IgG
- Antibody clone number
- 4D11-B3-E8
- Vial size
- 100 µl
- Concentration
- NULL
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot detection of COX IV in Goat muscle,CHO-k1,COS7,3T3,Hela,C6 and K562 cell lysates using COX IV mouse mAb (1:5000 diluted).Predicted band size:17KDa.Observed band size:17KDa.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot detection of COX IV in Mouse skeletal muscel and Jurkat lysates using COX IV mouse mAb (1:1000 diluted). Predicted band size: 17KDa.Observed band size: 17KDa.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunocytochemistry of HeLa cells using anti-COX IV mouse mAb diluted 1:150.
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunoprecipitation analysis of Hela cell lysates using COX IV mouse mAb.
- Validation comment
- IP
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human colorectal carcinoma with COX IV Mouse mAb (4D11-B3-E8,1:50 diluted),showing cytoplasm localization.A high pressure mediated antigen retrieval step was performed in citrate buffer(pH6.0).
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Flow Cytometry analysis of K562 cells stained with COX4 (red, 1/100 dilution), followed by FITC-conjugated goat anti-mouse IgG. Blue line histogram represents the isotype control, normal mouse IgG.
- Validation comment
- FC
