14-9775-80

antibody from Invitrogen Antibodies

Targeting: COX4I1 COX4, COX4-1

Western blot (Recommended by provider)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Recommended by provider)

Antibody Data

Product number

14-9775-80

Provider

Invitrogen Antibodies

Product name

Anti-COX4 Monoclonal Antibody (IB52C31H10), eBioscience™

Antibody type

Monoclonal

Antigen

Other

Description

Description: The IB52C31H10 monoclonal antibody recognizes human COX IV. COX IV, also known as cytochrome c oxidase (COX) or Complex IV, is the terminal complex of the electron transport chain. COX IV is encoded by nuclear DNA and localized to the inner mitochondrial membrane. COX IV functions in energy metabolism and shuttles electrons from cytochrome c to oxygen. Dysfunction in COX IV is associated with increased production of reactive oxygen species and cellular toxicity. Genetic defects in COX IV results in severe, often fatal metabolic disorders that primarily affect tissues with high energy demands such as heart, brain, and muscle. Applications Reported: This IB52C31H10 antibody has been reported for use in immunoprecipitation, western blotting, immunohistochemical staining of frozen tissue sections, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, microscopy, ELISA, and immunocytochemistry. Applications Tested: This IB52C31H10 antibody has been tested by immunocytochemistry of methanol-fixed human cells and can be used at less than or equal to 1 µg/mL. The IB52C31H10 antibody has also been tested by immunohistochemistry of formalin-fixed paraffin embedded tissue using low pH antigen retrieval and can be used at less than or equal to 1 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 µm post-manufacturing filtered.

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

IB52C31H10

Vial size

25 µg

Concentration

0.5 mg/mL

Storage

4° C

References

Submitted references

Cytochrome c oxidase dysfunction in oxidative stress.

Srinivasan S, Avadhani NG
Free radical biology & medicine 2012 Sep 15;53(6):1252-63

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Assembly of mitochondrial cytochrome c-oxidase, a complicated and highly regulated cellular process.

Fontanesi F, Soto IC, Horn D, Barrientos A
American journal of physiology. Cell physiology 2006 Dec;291(6):C1129-47

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Genetic defects of cytochrome c oxidase assembly.

Pecina P, Houstková H, Hansíková H, Zeman J, Houstek J
Physiological research 2004;53 Suppl 1:S213-23

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Functional and molecular mitochondrial abnormalities associated with a C --> T transition at position 3256 of the human mitochondrial genome. The effects of a pathogenic mitochondrial tRNA point mutation in organelle translation and RNA processing.

Hao H, Moraes CT
The Journal of biological chemistry 1996 Jan 26;271(4):2347-52

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry of fixed HeLa cells stained with 1 µg/mL of Mouse IgG1 K Isotype Control Purified (left) or 1 µg/mL of Anti-Human COX IV Purified (right) followed by F (ab')2 Anti-Mouse IgG eFluor® 570.Nuclei are stained with DAPI.