GTX628901

antibody from GeneTex

Targeting: COX4I1 COX4, COX4-1

Western blot (Enhanced validation)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

GTX628901

Provider

GeneTex

Product name

COX4 antibody [GT854]

Antibody type

Monoclonal

Reactivity

Human, Mouse, Rat

Host

Mouse

Western blot

Enhanced validation

Submitted by

GeneTex (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 15% SDS-PAGE, and the membrane was blotted with COX4 antibody [GT854] (GTX628901) diluted at 1:1500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

KD Reagent Type

siRNA

Downregulation

>50%

KD Reagent Provider

shRNA reagents was provided courtesy of cooperative labs.

KD Reagent Prod page

KD Reagent Prod page

KD Reagent Prod no

Proprietary.

Antibody Lot Number

41253

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Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

COX4 antibody [GT854] detects COX4I1 protein by western blot analysis.A. 30 ?g 293T whole cell lysate/extract B. 30 ?g A431 whole cell lysate/extractC. 30 ?g HeLa whole cell lysate/extractD. 30 ?g HepG2 whole cell lysate/extractE. 30 ?g A375 whole cell lysate/extract12% SDS-PAGECOX4 antibody [GT854] (GTX628901) dilution: 1:1000 The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

COX4 antibody detects COX4I1 protein by western blot analysis.A. 50 ?g mouse heart lysate/extract12% SDS-PAGECOX4 antibody (GTX628901) dilution: 1:1000 The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

COX4 antibody [GT854] detects COX4I1 protein by western blot analysis.A. 50 ?g rat muscle lysate/extract15% SDS-PAGECOX4 antibody [GT854] (GTX628901) dilution: 1:1000 The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 15% SDS-PAGE, and the membrane was blotted with COX4 antibody [GT854] (GTX628901) diluted at 1:1500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Immunocytochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

COX4 antibody [GT854] detects COX4 protein at cytoplasm by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 10 min.Green: COX4 protein stained by COX4 antibody [GT854] (GTX628901) diluted at 1:300.Blue: Hoechst 33342 staining.Scale bar = 10 £gm.