PA5-33597

antibody from Invitrogen Antibodies

Targeting: GNRHR GRHR, LHRHR

Immunohistochemistry (Recommended by provider)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-33597

Provider

Invitrogen Antibodies

Product name

GnRHR Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Percent identity with other species by BLAST analysis: Human, Gibbon (100%) Gorilla, Monkey, Dog, Elephant, Panda (95%) Marmoset, Bovine, Horse, Pig (90%).

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

50 µg

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Natural autoantibodies to the gonadotropin-releasing hormone receptor in polycystic ovarian syndrome.

Sattler LM, Schniewind HA, Minich WB, Haudum CW, Niklowitz P, Münzker J, Kovács GL, Reinehr T, Obermayer-Pietsch B, Schomburg L
PloS one 2021;16(4):e0249639

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig 2 Prevalence of GnRH-R-aAb in serum samples of controls and PCOS patients. A) The full set of samples (n = 1051) was analyzed for GnRH-R-aAb concentrations by measuring GnRH-R-Luc activity in the immunoprecipitates. Binding indices (BI) were calculated based on the lower half of all samples. The mean value plus three standard deviations (mean+3SD) was used as criterion for outliers, indicating positive presence of GnRH-R-aAb. Three samples were strongly positive, and 12 samples were slightly above the threshold (dashed line). B) The signals for GnRH-R-aAb were not normally distributed in the cohorts of samples analyzed. The prevalence of highly positive samples is similar in the cohorts of PCOS patients and control subjects (2 out of 651, i.e., 0.31%, vs. 1 out of 400, i.e., 0.25%).