Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [3]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- NBP1-22987 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-22987, RRID:AB_1726505
- Product name
- Rabbit Polyclonal MASTL Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references Fcp1 phosphatase controls Greatwall kinase to promote PP2A-B55 activation and mitotic progression.
Della Monica R, Visconti R, Cervone N, Serpico AF, Grieco D
eLife 2015 Dec 14;4
eLife 2015 Dec 14;4
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MASTL Antibody [NBP1-22987] - Whole cell lysate from HeLa and 293T cells. MASTL was also immunoprecipitated by another rabbit anti-MASTL antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MASTL Antibody [NBP1-22987] - Detection of Human MASTL by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MASTL antibody NBP1-22987 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MASTL Antibody [NBP1-22987] - Whole cell lysate (15 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MASTL antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: MASTL Antibody [NBP1-22987] - Detection of human MASTL by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-MASTL antibody NBP1-22987 (lot NBP1-22987-4) used for IP at 3 ug per reaction. MASTL was also immunoprecipitated by a previous lot of this antibody (lot NBP1-22987-3). For blotting immunoprecipitated MASTL, NBP1-22987 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: MASTL Antibody [NBP1-22987] - FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-MASTL used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB