Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [3]
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- Product number
- PA5-81568 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-eIF3g Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%). This antibody has specificity for Human EIF3S4.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of eIF3g in Lane A: PC12 Whole Cell Lysate (30 µg). Samples were probed using a eIF3g Polyclonal Antibody (Product # PA5-81568) at a 1:500 dilution, followed by a Goat Anti-Rabbit IgG (H+L), Dylight 800 Secondary Antibody at a 1:10000 dilution. Western blot was performed under reducing conditions. Predicted band size:36 kDa. Observed band size:34 kDa.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using eIF3g Polyclonal Antibody (Product # PA5-81568) at 1:500 dilution. Lane A: PC12 Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary antibody: Goat Anti- RabbitIgG H&L (DyLight™ 800) at 1:10,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 36 kDa. Observed band size: 34 kDa.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence staining of eIF3g in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with eIF3g Polyclonal Antibody (Product # PA5-81568, 1:5,000) at 4°C overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to cytoplasm and nucleus.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of eIF3g in human kidney with eIF3g Polyclonal Antibody (Product # PA5-81568, 1:5,000 dilution, formalin-fixed paraffin embedded sections).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of eIF3g in mouse kidney with eIF3g Polyclonal Antibody (Product # PA5-81568, 1:5,000 dilution, formalin-fixed paraffin embedded sections).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of eIF3g in rat kidney with eIF3g Polyclonal Antibody (Product # PA5-81568, 1:5,000 dilution, formalin-fixed paraffin embedded sections).