PA5-25261

antibody from Invitrogen Antibodies

Targeting: EIF3G eIF3-delta, eIF3-p44, EIF3S4

Western blot (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-25261

Provider

Invitrogen Antibodies

Product name

eIF3g Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

This antibody is predicted to react with bovine, mouse, rat and Xenopus and zebrafish based on sequence homology.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

400 µL

Concentration

0.25 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Human eIF3b and eIF3a serve as the nucleation core for the assembly of eIF3 into two interconnected modules: the yeast-like core and the octamer.

Wagner S, Herrmannová A, Šikrová D, Valášek LS
Nucleic acids research 2016 Dec 15;44(22):10772-10788

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry analysis of K562 cells using an EIF3G polyclonal antibody (Product # PA5-25261) (bottom) compared to a negative control cell (top) at a dilution of 1:10-50, followed by a FITC-conjugated goat anti-rabbit antibody

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 3. The octameric right leg subunits eIF3k and eIF3l impact their mutual expression and are dispensable for the integrity of the rest of eIF3, and the octameric right arm subunit eIF3e stabilizes binding of the right leg subunits (k, l) and eIF3d to the octamer, as well as the octamer attachment to the YLC. Protein levels of all eIF3 subunits and other eIFs upon knock down of either eIF3k, l or e were determined by Western blotting ( A, C, E , respectively). GAPDH was used as a loading control. To assess the integrity of eIF3, the anti-eIF3b co-immunoprecipitation experiments were carried out for each knock-down and the immunoprecipitated eIF3 subunits along with eIF3b were detected by Western blotting ( B, D, F ). The octameric eIF3 subunits are arranged at the left-hand side of each panel and non-octameric subunits are at the right-hand side. The YLC subunits are grouped and highlighted in bold. Quantifications of at least five independent experiments with standard deviations are shown in Supplementary Figure S4 and Table 1 .