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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
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- Product number
- GTX628545 - Provider product page
- Provider
- GeneTex
- Product name
- ADAR1 antibody [GT1066]
- Antibody type
- Monoclonal
- Host
- Mouse
- Vial size
- 100 µl
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- RFP antibody [GT1610] detects RFP (red fluorescent protein ) protein by Western blot analysis.A. 30µg 293T whole cell lysate/extractB. 30 µg whole cell lysate/extract of RFP-transfected 293T cells12 % SDS-PAGERFP antibody [GT1610] (GTX628545) dilution: 1:1000
- Validation comment
- WB
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- RFP-tag antibody detects RFP-recombinant protein by immunofluorescent analysis. Sample: RFP-tag transfected HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: RFP-recombinant protein stained by RFP-tag antibody (GTX628545) diluted at 1:2000. Red: RFP is expressed in the tranfected cell . Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- RFP tag antibody immunoprecipitates RFP-fused protein in IP experiments. IP Sample: 400 ?g cell lysate/extract of RFP-fused gene transfected 293T cells A. 30 ug lysate/extract of transfected 293T cell B. Control with 2 £gg of preimmune mouse IgG C. Immunoprecipitation of RFP-fused protein by 2 £gg of RFP tag antibody (GTX628545) 12% SDS-PAGE The immunoprecipitated RFP-fused protein was detected by RFP tag antibody (GTX628545) diluted at 1:1000. EasyBlot anti-nouse IgG (GTX221667-01) was used as a secondary reagent.
