PAB29440

antibody from Abnova Corporation

Targeting: ADAR ADAR1, G1P1, IFI4

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB29440

Provider

Abnova Corporation

Product name

ADAR polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant human ADAR.

Antigen sequence

ALLTHFLQPIYLKSVTLGYLFSQGHLTRAICCRVT
RDGSAFEDGLRHPFIVNHPKVGRVSIYDSKRQSGK
TKETSVNWCLADGYDLEILDGTRGTVDGPRNELSR
VSKKNIFLLFKKLCSFRYRRDLLRLSYGEAKKAAR
DY


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: Human cell line RT-4 Lane 2: Human cell line U-251MG with ADAR polyclonal antibody (Cat # PAB29440) at 1:500-1:1000 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-2 OS with ADAR polyclonal antibody (Cat # PAB29440) at 1-4 ug/mL concentration shows positivity in nucleoli and nucleus but excluded from the nucleoli.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human cerebral cortex with ADAR polyclonal antibody (Cat # PAB29440) shows strong nuclear positivity in neuronal cells at 1:1000-1:2500 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)