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Western blot
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ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- AMAb90535 - Provider product page
- Provider
- Atlas Antibodies
- Proper citation
- Atlas Antibodies Cat#AMAb90535, RRID:AB_2665578
- Product name
- Anti-ADAR
- Antibody type
- Monoclonal
- Description
- Monoclonal Antibody against Human ADAR, Clone ID: CL0176, Gene description: adenosine deaminase, RNA-specific, Alternative Gene Names: ADAR1, G1P1, IFI4, Validated applications: WB, IHC, ICC, Uniprot ID: P55265, Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Antibody clone number
- CL0176
- Vial size
- 100 µl
- Concentration
- 1.0 mg/ml
- Storage
- Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Handling
- The antibody solution should be gently mixed before use.
Submitted references The transcribed ultraconserved region uc.160+ enhances processing and A-to-I editing of the miR-376 cluster: hypermethylation improves glioma prognosis.
Metabolomic and Lipidomic Profiling Identifies The Role of the RNA Editing Pathway in Endometrial Carcinogenesis
Gene amplification-associated overexpression of the RNA editing enzyme ADAR1 enhances human lung tumorigenesis
Soler M, Davalos V, Sánchez-Castillo A, Mora-Martinez C, Setién F, Siqueira E, Castro de Moura M, Esteller M, Guil S
Molecular oncology 2022 Feb;16(3):648-664
Molecular oncology 2022 Feb;16(3):648-664
Metabolomic and Lipidomic Profiling Identifies The Role of the RNA Editing Pathway in Endometrial Carcinogenesis
Altadill T, Dowdy T, Gill K, Reques A, Menon S, Moiola C, Lopez-Gil C, Coll E, Matias-Guiu X, Cabrera S, Garcia A, Reventos J, Byers S, Gil-Moreno A, Cheema A, Colas E
Scientific Reports 2017;7(1)
Scientific Reports 2017;7(1)
Gene amplification-associated overexpression of the RNA editing enzyme ADAR1 enhances human lung tumorigenesis
Anadón C, Guil S, Simó-Riudalbas L, Moutinho C, Setien F, Martínez-Cardús A, Moran S, Villanueva A, Calaf M, Vidal A, Lazo P, Zondervan I, Savola S, Kohno T, Yokota J, Ribas de Pouplana L, Esteller M
Oncogene 2015;35(33):4407-4413
Oncogene 2015;35(33):4407-4413
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Enhanced validation
- Submitted by
- Atlas Antibodies (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-ADAR antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
- Sample type
- Human
- Protocol
- Protocol
Supportive validation
- Submitted by
- Atlas Antibodies (provider)
- Enhanced method
- Orthogonal validation
- Main image
- Experimental details
- Immunohistochemistry analysis in human cerebral cortex and skeletal muscle tissues using AMAb90535 antibody. Corresponding ADAR RNA-seq data are presented for the same tissues.
- Sample type
- Human
- Protocol
- Protocol
