Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [6]
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Validation data
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- Product number
- PA5-54843 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CDH17 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: INNVMYFQIN NKTGAISLTR EGSQELNPAK NPSYNLVISV KDMGGQSENS FSDTTSVDII VTENIWKAPK PVEMVENSTD P Highest antigen sequence identity to the following orthologs: Mouse - 83%, Rat - 83%.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.2 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CDH17 in control (vector only transfected HEK293T lysate) and CDH17 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells). Samples were probed using a CDH17 Polyclonal Antibody (Product # PA5-54843).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CDH17 in human cell line CACO-2 using a CDH17 Polyclonal Antibody (Product # PA5-54843).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CDH17 in control (vector only transfected HEK293T lysate) and CDH17 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells). Samples were probed using a CDH17 Polyclonal Antibody (Product # PA5-54843).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of CDH17 in human cell line A549 shows positivity in nucleus but excluded from the nucleoli. Samples were probed using a CDH17 Polyclonal Antibody (Product # PA5-54843).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of CDH17 in human colon using CDH17 Polyclonal Antibody (Product # PA5-54843).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of CDH17 in human kidney using CDH17 Polyclonal Antibody (Product # PA5-54843).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of CDH17 in human duodenum and liver tissues using CDH17 Polyclonal Antibody (Product # PA5-54843). Corresponding CDH17 RNA-seq data are presented for the same tissues.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of CDH17 in human colon, duodenum, kidney and liver using CDH17 Polyclonal Antibody (Product # PA5-54843) (A) shows similar protein distribution across tissues to an independent CDH17 Polyclonal Antibody (B).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of CDH17 in human duodenum using CDH17 Polyclonal Antibody (Product # PA5-54843) shows high expression.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of CDH17 in human liver using CDH17 Polyclonal Antibody (Product # PA5-54843) shows low expression as expected.