NBP1-88237

antibody from Novus Biologicals

Targeting: CDH17 cadherin, HPT-1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP1-88237

Provider

Novus Biologicals

Proper citation

Novus Cat#NBP1-88237, RRID:AB_11027899

Product name

Rabbit Polyclonal Cadherin-17 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Specificity of human Cadherin-17 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

0.1 ml

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Cadherin-17 Antibody [NBP1-88237] - Analysis in human cell lines Caco-2 and SK-MEL-30 using anti-CDH17 antibody. Corresponding CDH17 RNA-seq data are presented for the same cell lines. Loading control: anti-HDAC1.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Cadherin-17 Antibody [NBP1-88237] - Analysis in control (vector only transfected HEK293T lysate) and CDH17 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Cadherin-17 Antibody [NBP1-88237] - Staining of human colon shows strong cytoplasmic and membranous positivity in glandular cells.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Cadherin-17 Antibody [NBP1-88237] - Staining of human duodenum shows high expression.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Cadherin-17 Antibody [NBP1-88237] - Staining of human liver shows low expression as expected.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Cadherin-17 Antibody [NBP1-88237] - Staining in human duodenum and liver tissues using anti-CDH17 antibody. Corresponding CDH17 RNA-seq data are presented for the same tissues.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Cadherin-17 Antibody [NBP1-88237] - Staining of human colon, duodenum, kidney and liver using Anti-CDH17 antibody NBP1-88237 (A) shows similar protein distribution across tissues to independent antibody NBP1-88239 (B).

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Cadherin-17 Antibody [NBP1-88237] - Staining of human kidney.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Cadherin-17 Antibody [NBP1-88237] - Staining of human colon.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Cadherin-17 Antibody [NBP1-88237] - Staining of human duodenum using Anti-CDH17 antibody NBP1-88237.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Cadherin-17 Antibody [NBP1-88237] - Staining of human liver using Anti-CDH17 antibody NBP1-88237.