Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [9]
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Validation data
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- Product number
- NBP1-88238 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-88238, RRID:AB_11033916
- Product name
- Rabbit Polyclonal Cadherin-17 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human Cadherin-17 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references SATB2 in combination with cytokeratin 20 identifies over 95% of all colorectal carcinomas.
Magnusson K, de Wit M, Brennan DJ, Johnson LB, McGee SF, Lundberg E, Naicker K, Klinger R, Kampf C, Asplund A, Wester K, Gry M, Bjartell A, Gallagher WM, Rexhepaj E, Kilpinen S, Kallioniemi OP, Belt E, Goos J, Meijer G, Birgisson H, Glimelius B, Borrebaeck CA, Navani S, Uhlén M, O'Connor DP, Jirström K, Pontén F
The American journal of surgical pathology 2011 Jul;35(7):937-48
The American journal of surgical pathology 2011 Jul;35(7):937-48
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Cadherin-17 Antibody [NBP1-88238] - Analysis in human cell lines Caco-2 and A-431 using anti-CDH17 antibody. Corresponding CDH17 RNA-seq data are presented for the same cell lines. Loading control: anti-PPIB.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Cadherin-17 Antibody [NBP1-88238] - Analysis in control (vector only transfected HEK293T lysate) and CDH17 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Cadherin-17 Antibody [NBP1-88238] - Staining of human rectum shows strong cytoplasmic and membranous positivity in glandular cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Cadherin-17 Antibody [NBP1-88238] - Staining of human duodenum shows strong membranous positivity in glandular cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Cadherin-17 Antibody [NBP1-88238] - Staining of human liver shows low expression as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Cadherin-17 Antibody [NBP1-88238] - Staining in human duodenum and liver tissues using anti-CDH17 antibody. Corresponding CDH17 RNA-seq data are presented for the same tissues.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Cadherin-17 Antibody [NBP1-88238] - Staining of human colon, duodenum, kidney and liver using Anti-CDH17 antibody NBP1-88238 (A) shows similar protein distribution across tissues to independent antibody NBP1-88239 (B).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Cadherin-17 Antibody [NBP1-88238] - Staining of human colon.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Cadherin-17 Antibody [NBP1-88238] - Staining of human kidney.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Cadherin-17 Antibody [NBP1-88238] - Staining of human duodenum using Anti-CDH17 antibody NBP1-88238.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Cadherin-17 Antibody [NBP1-88238] - Staining of human liver using Anti-CDH17 antibody NBP1-88238.