Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- AF5529 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal Eps8 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects endogenous human Eps8 in Western blots.
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human Eps8 by Western Blot. Western blot shows lysates of A431 human epithelial carcinoma cell line, MCF-7 human breast cancer cell line, MDA-MB-468 human breast cancer cell line, and A549 human lung carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Human Eps8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5529) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Eps8 at approximately 97 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.
