R31674

antibody from NSJ Bioreagents

Targeting: MCM7 CDC47, MCM2, PPP1R104

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

R31674

Provider

NSJ Bioreagents

Product name

MCM7 Antibody

Antibody type

Polyclonal

Description

This highly specific MCM7 antibody is suitable for use in Western blot/Immunohistochemistry/Immunocytochemistry/Immunofluorescence/Flow cytometry applications with human, mouse and rat samples.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Vial size

100 ug

Concentration

0.5mg/ml if reconstituted with 0.2ml sterile DI water

Storage

The lyophilized MCM7 antibody can be stored at 4oC to -20oC. After reconstitution, aliquot and store at -20oC. Avoid repeated freeze/thaws.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of MCM7 antibody and Lane 1: rat brain; 2: human placenta; 3: mouse NIH3T3; 4: (h) HeLa; 5: (h) Jurkat; 6: (h) 22RV1; 7: (h) COLO320; 8: (r) PC12 lysate. Expected size 80~90KD

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of MCM7 antibody and recombinant human protein (0.5ng)

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of 1) human HeLa, 2) human K562, 3) human Jurkat, 4) human MCF7, 5) rat C6 and 6) mouse Neuro-2a cell lysate with MCM7 antibody. Expected molecular weight: 80-90 kDa.

Immunocytochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

ICC testing of MCM7 antibody and A549 cells

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Immunofluorescent staining of FFPE human colon cancer tissue with MCM7 antibody. HIER: steam section in pH6 citrate buffer for 20 min.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Immunofluorescent staining of FFPE human colon cancer tissue with MCM7 antibody. HIER: steam section in pH6 citrate buffer for 20 min.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Immunofluorescent staining of FFPE human U-2 OS cells with MCM7 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Immunocytochemical staining of FFPE human A549 cells with MCM7 antibody. HIER: steam section in pH6 citrate buffer for 20 min.

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC-P: MCM7 antibody testing of human lung cancer tissue

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC-P testing of mouse testis tissue

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC-P testing of rat intestine tissue

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE human lung cancer tissue with MCM7 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE mouse testis tissue with MCM7 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE rat intestinal tissue with MCM7 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of human A431 cells with MCM7 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= MCM7 antibody.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of human U937 cells with MCM7 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= MCM7 antibody.