NBP1-31350
antibody from Novus Biologicals
Targeting: MCM7
CDC47, MCM2, PPP1R104
Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Chromatin Immunoprecipitation [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- NBP1-31350 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-31350, RRID:AB_10003785
- Product name
- Rabbit Polyclonal MCM7 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Cyclin-dependent kinases regulate lysosomal degradation of hypoxia-inducible factor 1α to promote cell-cycle progression.
A nontranscriptional role for HIF-1α as a direct inhibitor of DNA replication.
MCM proteins are negative regulators of hypoxia-inducible factor 1.
Hubbi ME, Gilkes DM, Hu H, Kshitiz, Ahmed I, Semenza GL
Proceedings of the National Academy of Sciences of the United States of America 2014 Aug 12;111(32):E3325-34
Proceedings of the National Academy of Sciences of the United States of America 2014 Aug 12;111(32):E3325-34
A nontranscriptional role for HIF-1α as a direct inhibitor of DNA replication.
Hubbi ME, Kshitiz, Gilkes DM, Rey S, Wong CC, Luo W, Kim DH, Dang CV, Levchenko A, Semenza GL
Science signaling 2013 Feb 12;6(262):ra10
Science signaling 2013 Feb 12;6(262):ra10
MCM proteins are negative regulators of hypoxia-inducible factor 1.
Hubbi ME, Luo W, Baek JH, Semenza GL
Molecular cell 2011 Jun 10;42(5):700-12
Molecular cell 2011 Jun 10;42(5):700-12
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MCM7 Antibody [NBP1-31350] - Sample (30 ug of whole cell lysate) A: H1299 7. 5% SDS PAGE; antibody diluted at 1:1000.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: MCM7 Antibody [NBP1-31350] - Paraffin-embedded Cal27 Xenograft, using antibody at 1:100 dilution.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Chromatin Immunoprecipitation (ChIP): MCM7 Antibody [NBP1-31350] - Cross-linked ChIP was performed with HeLa chromatin extract and 5 ug of either control rabbit igG or anti-MCM7 antibody. The precipitated DNA was detected by PCR with primer set targeting to Myc promoter.