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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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Validation data
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- Product number
- 200-501-F39 - Provider product page
- Provider
- Rockland Immunochemicals, Inc.
- Proper citation
- Rockland Cat#200-501-F39, RRID:AB_2612532
- Product name
- Anti-GRP94 (RAT) Monoclonal Antibody - 200-501-F39
- Antibody type
- Monoclonal
- Vial size
- 200 µl
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Supportive validation
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- Rockland Immunochemicals, Inc. (provider)
- Main image
- Experimental details
- Western Blot of rat anti-Grp94 antibody. Lane 1: molecular weight. Lane 2: HeLa Lysates. Load: 35 µg per lane. Primary antibody: Grp94 antibody at 1:1000 for overnight at 4°C. Secondary antibody: IRDye800 rat secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 92.5 kDa, ~110 kDa for Grp94. Other band(s): none.
- Validation comment
- Western Blot
