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LearnPA5-28221
antibody from Invitrogen Antibodies
Targeting: ZEB1
AREB6, BZP, FECD6, NIL-2-A, PPCD3, TCF8, ZEB, Zfhep, Zfhx1a
Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [6]
- Immunocytochemistry [1]
- Chromatin Immunoprecipitation [1]
- Other assay [4]
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- Product number
- PA5-28221 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ZEB1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: HeLa, 293T, HepG2, MDA-MB-231, Neuro2A, NIH-3T3, C2C12, ZEB1 shRNA-transfected HeLa. Predicted reactivity: Mouse (91%), Rat (89%), Chicken (90%), Bovine (95%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Schlafen12 Reduces the Aggressiveness of Triple Negative Breast Cancer through Post-Transcriptional Regulation of ZEB1 That Drives Stem Cell Differentiation.
Paracrine signalling during ZEB1-mediated epithelial-mesenchymal transition augments local myofibroblast differentiation in lung fibrosis.
Al-Marsoummi S, Vomhof-DeKrey E, Basson MD
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 2019;53(6):999-1014
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 2019;53(6):999-1014
Paracrine signalling during ZEB1-mediated epithelial-mesenchymal transition augments local myofibroblast differentiation in lung fibrosis.
Yao L, Conforti F, Hill C, Bell J, Drawater L, Li J, Liu D, Xiong H, Alzetani A, Chee SJ, Marshall BG, Fletcher SV, Hancock D, Coldwell M, Yuan X, Ottensmeier CH, Downward J, Collins JE, Ewing RM, Richeldi L, Skipp P, Jones MG, Davies DE, Wang Y
Cell death and differentiation 2019 May;26(5):943-957
Cell death and differentiation 2019 May;26(5):943-957
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of AREB6 using 30 µg of A) 293T and B) HeLa lysate. Samples were loaded onto a 5% SDS-PAGE gel and probed with an AREB6 polyclonal antibody (Product # PA5-28221) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot was performed using Anti-ZEB1 Polyclonal Antibody (Product # PA5-28221) and a 170 kDa band corresponding to ZEB1 was observed across cell lines tested. Whole cell extracts (30 µg lysate) of MDA-MB-231 (Lane 1), MCF7 (Lane 2), LNCaP (Lane 3), T-47D (Lane 4) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 Dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).Expression of ZEB-1 was found higher in MDA-MB-231 as compared to MCF-7, LNCaP and T-47D.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of ZEB1 was performed by separating 30 µg of various whole cell extracts by 5% SDS-PAGE. Proteins were transferred to a membrane and probed with a ZEB1 Polyclonal Antibody (Product # PA5-28221) at a dilution of 1:1000 and a HRP-conjugated anti-rabbit IgG secondary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of ZEB1 was performed by separating 30 µg of various whole cell extracts by 5% SDS-PAGE. Proteins were transferred to a membrane and probed with a ZEB1 Polyclonal Antibody (Product # PA5-28221) at a dilution of 1:1000 and a HRP-conjugated anti-rabbit IgG secondary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of ZEB1 was performed by separating 30 µg of various whole cell extracts by 5% SDS-PAGE. Proteins were transferred to a membrane and probed with a ZEB1 Polyclonal Antibody (Product # PA5-28221) at a dilution of 1:1000 and a HRP-conjugated anti-rabbit IgG secondary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot was performed using Anti-ZEB1 Polyclonal Antibody (Product # PA5-28221) and a 170 kDa band corresponding to ZEB1 was observed across cell lines tested. Whole cell extracts (30 µg lysate) of MDA-MB-231 (Lane 1), MCF7 (Lane 2), LNCaP (Lane 3), T-47D (Lane 4) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 Dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).Expression of ZEB-1 was found higher in MDA-MB-231 as compared to MCF-7, LNCaP and T-47D.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of ZEB1 was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: ZEB1 Polyclonal Antibody (Product # PA5-28221) diluted at 1:500. Red: Phalloidin, a cytoskeleton marker. Scale bar = 10 µm.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Cross-linked ChIP was performed with HeLa chromatin extract and 5 µg of either control rabbit IgG or ZEB1 Polyclonal Antibody (Product # PA5-28221). The precipitated DNA was detected by PCR with primer set targeting to LAMC2 promotor.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ZEB1 antibody immunoprecipitates ZEB1 protein in IP experiments. IP Sample: 293T whole cell lysate/extract A. 40 µg 293T whole cell lysate/extract B. Control with 2 µg of preimmune rabbit IgG C. Immunoprecipitation of ZEB1 protein by 2 µg of ZEB1 antibody (Product # PA5-28221) 7.5% SDS-PAGE The immunoprecipitated ZEB1 protein was detected by ZEB1 antibody (Product # PA5-28221) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 7 ZEB1 regulates the expression of tissue plasminogen activator (tPA), which acts as a paracrine regulator of TGFbeta-induced fibroblast activation. a Increased expression of PLAT (tPA) in IPF epithelial cells is shown by an online LGEA web portal ( https://research.cchmc.org/pbge/lunggens/mainportal.html ). b Quantitative secretome analysis identifies an increased level of tPA in the conditioned media (CM) from 4-OHT-treated ATII ER:KRASV12 cells and a representative tPA Western blot of CM from control or 4-OHT-treated ATII ER:KRASV12 cells. Data are individual values with mean and s.d. n = 3 samples per group. Values were normalised to total fmol of each sample multiplied by 10,000. c Fold change in mRNA levels of ZEB1 and PLAT (tPA) in ATII ER:KRASV12 cells with indicated treatments. beta-actin-normalised mRNA levels in control cells were used to set the baseline value at unity. Data are mean +- s.d. n = 3 samples per group. *** P < 0.001. d ChIP assays of ZEB1's ability to bind the PLAT (tPA) promoter in ATII ER:KRASV12 cells with indicated treatments. The amplified PLAT (tPA) promoter region (-547 to -345) contains a ZEB1 binding site at -419. Values represent relative binding in relation to input (2%), normalised against control (1.0). Data are mean +- s.d. n = 4 samples per group. *** P < 0.001. e PLAT promoter reporter assays in ATII ER:KRASV12 cells with indicated treatments. Values represent relative fold of firefly luciferase in relation to Renilla luciferase,
