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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunocytochemistry [1]
- Other assay [2]
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- Product number
- PA5-34687 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- BIN1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: A431, mouse kidney. Predicted reactivity: Mouse (92%), Rat (80%), Pig (92%), Chicken (81%), Bovine (92%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1.34 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Upregulation of RIN3 induces endosomal dysfunction in Alzheimer's disease.
Shen R, Zhao X, He L, Ding Y, Xu W, Lin S, Fang S, Yang W, Sung K, Spencer B, Rissman RA, Lei M, Ding J, Wu C
Translational neurodegeneration 2020 Jun 18;9(1):26
Translational neurodegeneration 2020 Jun 18;9(1):26
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- BIN1 Polyclonal Antibody detects BIN1 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: BIN1 stained by BIN1 Polyclonal Antibody (Product # PA5-34687) diluted at 1:500. Blue: Fluoroshield with DAPI . Scale bar= 10 µm.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 4 RIN3 colocalizes with CD2AP and BIN1. PC12M cells ( a and b ) and primary cortical neurons ( c and d ) were transfected with RIN3-GFP, followed by immunostaining for CD2AP ( a , c ) or BIN1 ( b , d ) or CD2AP using specific antibodies. Yellow color denotes colocalization. Representative images are shown
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 7 BIN1 and CD2AP expression level in APP/PS1 mice. 3-month-old APP/PS1 mice and age-matched WT mice were dissected, lysates were immunoblotted for BIN1 and CD2AP ( a ). The protein levels were normalized against GAPDH as a loading control ( b ). In Cortex ( c ) and hippocampus ( d ) were also extracted from10-month-old APP/PS1 and age-matched WT mice. Protein lysates were used to probe for BIN1, CD2AP, RIN3, Rabex5 and Rab5 using indicated antibodies by SDS-PAGE/immunoblotting ( c and d ), The intensity of bands was quantitated using BioRad-ImageLab. The respective protein levels were normalized against GAPDH as a loading control ( e , f ). p < 0.05 (*), p < 0.01(**), p < 0.0001 (****), standard t-test
