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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
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- Product number
- TA319580 - Provider product page
- Provider
- OriGene
- Product name
- Mouse monoclonal anti-BIN1 antibody
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal anti-BIN1 antibody
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- BIN1
- Antibody clone number
- 99F
- Vial size
- 100 µg
- Concentration
- NULL
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western Blot of Mouse Anti-BIN1 antibody. Lane 1: C2C12 during growth. Lane 2: C2C12 during differentiation. Load: 35 ug per lane. Primary antibody: BIN1 antibody at 1:400 for overnight at 4°C. Secondary antibody: IRDye800? mouse secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 64.7 kDa, ~55 kDa for BIN-1. Other band(s): non-specifics.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunofluorescence Microscopy of Mouse Anti-BIN1 Antibody. Cells: C2C12 cells during growth or differentiation. Fixation: 0.5% PFA. Antigen retrieval: not required. Primary antibody: BIN-1 (Exon 10 specific, 99F) monoclonal antibody. Secondary antibody: mouse secondary antibody at 1:10,000 for 45 min at RT. Localization: BIN1 is nuclear and cytoplasmic. Staining: BIN 1 as green fluorescent signal.
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- IP of Mouse anti-BIN1 antibody. IP were separated by SDS-PAGE and visualized by fluorography. The differentiation-associated (Exon 10+) form of Bin1 is recognized by using Bin1 (Exon 10 specific, 99F) monoclonal antibody. Lane 1: BIN 1 Lane 2: none. Load: 35 ug per lane. Primary antibody: BIN-1 (Exon 10 specific, 99F) monoclonal antibody at 1:400 for overnight at 4°C. Secondary antibody: IRDye800? mouse secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C.
- Validation comment
- IP
