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- Antibody Data
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- References [1]
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- Western blot [2]
- Immunohistochemistry [3]
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- Product number
- NBP1-89102 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-89102, RRID:AB_11004165
- Product name
- Rabbit Polyclonal BIN1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human BIN1 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Immunofluorescence and fluorescent-protein tagging show high correlation for protein localization in mammalian cells.
Stadler C, Rexhepaj E, Singan VR, Murphy RF, Pepperkok R, Uhlén M, Simpson JC, Lundberg E
Nature methods 2013 Apr;10(4):315-23
Nature methods 2013 Apr;10(4):315-23
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: BIN1 Antibody [NBP1-89102] - Analysis in control (vector only transfected HEK293T lysate) and BIN1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: BIN1 Antibody [NBP1-89102] - Analysis in human cell lines Caco-2 and MCF-7 using anti-BIN1 antibody. Corresponding BIN1 RNA-seq data are presented for the same cell lines. Loading control: anti-PPIB.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: BIN1 Antibody [NBP1-89102] - Staining of human skeletal muscle shows strong cytoplasmic positivity in myocytes.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: BIN1 Antibody [NBP1-89102] - Staining of human heart muscle shows low expression as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: BIN1 Antibody [NBP1-89102] - Staining in human skeletal muscle and heart muscle tissues using anti-BIN1 antibody. Corresponding BIN1 RNA-seq data are presented for the same tissues.
