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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
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- Product number
- PA5-30176 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-BAF57 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: IMR32, Jurkat, NIH-3T3. Predicted reactivity: Mouse (100%), Rat (100%), Zebrafish (93%), Xenopus laevis (97%), Chicken (100%), Rhesus Monkey (100%), Bovine (100%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.43 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of BAF57 using 30 µg of NIH-3T3 lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a BAF57 polyclonal antibody (Product # PA5-30176) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of BAF57 using 30 µg of A) IMR32 and B) Jurkat lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a BAF57 polyclonal antibody (Product # PA5-30176) at a dilution of 1:1000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of BAF57 showing staining in the nucleus of MCF-7 cells. MCF-7 cells were fixed in 4% paraformaldehyde at RT for 15 min and stained using a BAF57 polyclonal antibody (Product # PA5-30176) diluted at 1:500. Blue: Hoechst 33343 staining.
