HPA003916

antibody from Atlas Antibodies

Targeting: SMARCE1 BAF57

Western blot (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Chromatin Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

HPA003916

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA003916, RRID:AB_1857279

Product name

Anti-SMARCE1

Antibody type

Polyclonal

Description

Polyclonal Antibody against Human SMARCE1, Gene description: SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily e, member 1, Alternative Gene Names: BAF57, Validated applications: ICC, IHC, ChIP, WB, Uniprot ID: Q969G3, Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

100 µl

Concentration

0.2 mg/ml

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Handling

The antibody solution should be gently mixed before use.

References

Submitted references

Is location more determining than WHO grade for long-term clinical outcome in patients with meningioma in the first two decades of life?

Hirschmann D, Nasiri D, Entenmann C, Haberler C, Roetzer T, Dorfer C, Millesi M
Wiener klinische Wochenschrift 2024;137(1-2):21-30

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Clear cell meningiomas are defined by a highly distinct DNA methylation profile and mutations in SMARCE1

Sievers P, Sill M, Blume C, Tauziede-Espariat A, Schrimpf D, Stichel D, Reuss D, Dogan H, Hartmann C, Mawrin C, Hasselblatt M, Stummer W, Schick U, Hench J, Frank S, Ketter R, Schweizer L, Schittenhelm J, Puget S, Brandner S, Jaunmuktane Z, Küsters B, Abdullaev Z, Pekmezci M, Snuderl M, Ratliff M, Herold-Mende C, Unterberg A, Aldape K, Ellison D, Wesseling P, Reifenberger G, Wick W, Perry A, Varlet P, Pfister S, Jones D, von Deimling A, Sahm F
Acta Neuropathologica 2020;141(2):281-290

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Loss of SMARCE1 expression is a specific diagnostic marker of clear cell meningioma: a comprehensive immunophenotypical and molecular analysis

Tauziede‐Espariat A, Parfait B, Besnard A, Lacombe J, Pallud J, Tazi S, Puget S, Lot G, Terris B, Cohen J, Vidaud M, Figarella‐Branger D, Monnien F, Polivka M, Adle‐Biassette H, Varlet P
Brain Pathology 2017;28(4):466-474

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Immunofluorescence and fluorescent-protein tagging show high correlation for protein localization in mammalian cells

Stadler C, Rexhepaj E, Singan V, Murphy R, Pepperkok R, Uhlén M, Simpson J, Lundberg E
Nature Methods 2013;10(4):315-323

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Systematic validation of antibody binding and protein subcellular localization using siRNA and confocal microscopy

Stadler C, Hjelmare M, Neumann B, Jonasson K, Pepperkok R, Uhlén M, Lundberg E
Journal of Proteomics 2012;75(7):2236-2251

Immunohistochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Orthogonal validation

Main image

Experimental details

Immunohistochemistry analysis in human endometrium and liver tissues using Anti-SMARCE1 antibody. Corresponding SMARCE1 RNA-seq data are presented for the same tissues.

Sample type

Human

Protocol

Protocol

Orthogonal method

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

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Chromatin Immunoprecipitation

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

ChIP-Exo-Seq composite graph for Anti-SMARCE1 (HPA003916, Lot A107052) tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.