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Learn210-501-B54S
antibody from Rockland Immunochemicals, Inc.
Targeting: IL27
IL-27, IL-27A, IL27A, IL27p28, IL30, MGC71873, p28
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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- Product number
- 210-501-B54S - Provider product page
- Provider
- Rockland Immunochemicals, Inc.
- Proper citation
- Rockland Cat#210-501-B54S, RRID:AB_11182354
- Product name
- Anti-Mouse IL-27/p28 (RAT) Monoclonal Antibody - 210-501-B54S
- Antibody type
- Monoclonal
- Vial size
- 25 µl
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Supportive validation
- Submitted by
- Rockland Immunochemicals, Inc. (provider)
- Main image
- Experimental details
- Anti-IL-27/p28 antibody in western blot shows detection of recombinant mouse IL-27/p28. Recombinant protein (0.1 µg) was loaded on to an SDS-PAGE gel, and after separation, transferred to nitrocellulose. The membrane was blocked with 1% BSA in TBST for 30 min at RT, followed by incubation with Rockland's Anti-IL-27/p28 antibody (p/n 210-501-B54) diluted 1:1,000 in 1% BSA in TBST overnight at 4°C. After washes, the blot was reacted with secondary antibody HRP Goat anti-Rat IgG antibody diluted 1:40,000 in blocking buffer (p/n MB-070) for 30 min at RT. Data was collected using Bio-Rad VersaDoc® 4000 MP imaging system. Band indicates the correct molecular weight of 26 kDa expected for Mouse IL-27/p28.
- Validation comment
- Western Blot
