60160-1-Ig

antibody from Proteintech Group

Targeting: FUS ALS6, FUS1, hnRNP-P2, HNRNPP2, TLS

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

60160-1-Ig

Provider

Proteintech Group

Proper citation

Proteintech Cat#60160-1-Ig, RRID:AB_10666169

Product name

FUS/TLS antibody

Antibody type

Monoclonal

Description

KD/KO validated FUS/TLS antibody (Cat. #60160-1-Ig) is a mouse monoclonal antibody that shows reactivity with human, mouse, rat, pig and has been validated for the following applications: FC, IF, IHC, IP, ELISA.

Reactivity

Human, Mouse, Rat, Porcine

Host

Mouse

Conjugate

Unconjugated

Isotype

IgG

Antibody clone number

3A10B5

Vial size

20ul, 150ul

References

Submitted references

Annexin A11 aggregation in FTLD-TDP type C and related neurodegenerative disease proteinopathies.

Robinson JL, Suh E, Xu Y, Hurtig HI, Elman L, McMillan CT, Irwin DJ, Porta S, Van Deerlin VM, Lee EB
Acta neuropathologica 2024 Jun 19;147(1):104

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A model of human neural networks reveals NPTX2 pathology in ALS and FTLD.

Hruska-Plochan M, Wiersma VI, Betz KM, Mallona I, Ronchi S, Maniecka Z, Hock EM, Tantardini E, Laferriere F, Sahadevan S, Hoop V, Delvendahl I, Pérez-Berlanga M, Gatta B, Panatta M, van der Bourg A, Bohaciakova D, Sharma P, De Vos L, Frontzek K, Aguzzi A, Lashley T, Robinson MD, Karayannis T, Mueller M, Hierlemann A, Polymenidou M
Nature 2024 Feb;626(8001):1073-1083

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The identification of high-performing antibodies for RNA-binding protein FUS for use in Western Blot, immunoprecipitation, and immunofluorescence.

Alshalfie W, Fotouhi M, Ayoubi R, You Z, Southern K, McPherson PS, Laflamme C, NeuroSGC/YCharOS/EDDU collaborative group
F1000Research 2023;12:376

---

Ballooned neurons in semi-recent severe traumatic brain injury.

Michaud J, Plu I, Parai J, Bourgault A, Tanguay C, Seilhean D, Woulfe J
Acta neuropathologica communications 2023 Mar 10;11(1):37

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Regulation of RNG105/caprin1 dynamics by pathogenic cytoplasmic FUS and TDP-43 in neuronal RNA granules modulates synaptic loss.

Horio T, Ishikura Y, Ohashi R, Shiina N
Heliyon 2023 Jun;9(6):e17065

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A Wnt-induced lncRNA-DGCR5 splicing switch drives tumor-promoting inflammation in esophageal squamous cell carcinoma.

Li Y, Chen B, Jiang X, Li Y, Wang X, Huang S, Wu X, Xiao Y, Shi D, Huang X, He L, Chen X, Ouyang Y, Li J, Song L, Lin C
Cell reports 2023 Jun 27;42(6):112542

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Patients carrying the mutation p.R406W in MAPT present with non-conforming phenotypic spectrum.

Gossye H, Van Mossevelde S, Sieben A, Bjerke M, Hendrickx Van de Craen E, van der Zee J, De Deyn PP, De Bleecker J, Versijpt J, van den Ende J, Deryck O, Bourgeois P, Bier JC, Goethals M, Vandenberghe R, Engelborghs S, Van Broeckhoven C
Brain : a journal of neurology 2023 Apr 19;146(4):1624-1636

---

Hyperosmotic-stress-induced liquid-liquid phase separation of ALS-related proteins in the nucleus.

Gao C, Gu J, Zhang H, Jiang K, Tang L, Liu R, Zhang L, Zhang P, Liu C, Dai B, Song J
Cell reports 2022 Jul 19;40(3):111086

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A novel promoter-associated non-coding small RNA paGLI1 recruits FUS/P65 to transactivate GLI1 gene expression and promotes infiltrating glioma progression.

Zhong J, Xu M, Su Z, Zhang M, Yu T, Nie L, Gong J, Chen X, Chen N, Zhou Q
Cancer letters 2022 Apr 1;530:68-84

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Hippocampal Sclerosis in Frontotemporal Dementia: When Vascular Pathology Meets Neurodegeneration.

Sieben A, Van Langenhove T, Vermeiren Y, Gossye H, Praet M, Vanhauwaert D, Cousaert C, Engelborghs S, Raedt R, Boon P, Santens P, De Deyn PP, Bracke KR, De Meulemeester K, Van Broeckhoven C, Martin JJ, Bjerke M
Journal of neuropathology and experimental neurology 2021 Mar 22;80(4):313-324

---

RBM45 associates with nuclear stress bodies and forms nuclear inclusions during chronic cellular stress and in neurodegenerative diseases.

Collins M, Li Y, Bowser R
Acta neuropathologica communications 2020 Jun 26;8(1):91

---

Self-assembly of FUS through its low-complexity domain contributes to neurodegeneration.

Matsumoto T, Matsukawa K, Watanabe N, Kishino Y, Kunugi H, Ihara R, Wakabayashi T, Hashimoto T, Iwatsubo T
Human molecular genetics 2018 Apr 15;27(8):1353-1365

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Motor neuron degeneration in spastic paraplegia 11 mimics amyotrophic lateral sclerosis lesions.

Denora PS, Smets K, Zolfanelli F, Ceuterick-de Groote C, Casali C, Deconinck T, Sieben A, Gonzales M, Zuchner S, Darios F, Peeters D, Brice A, Malandrini A, De Jonghe P, Santorelli FM, Stevanin G, Martin JJ, El Hachimi KH
Brain : a journal of neurology 2016 Jun;139(Pt 6):1723-34

---

A novel missense mutation of CMT2P alters transcription machinery.

Hu B, Arpag S, Zuchner S, Li J
Annals of neurology 2016 Dec;80(6):834-845

---

PINK1 and Parkin are genetic modifiers for FUS-induced neurodegeneration.

Chen Y, Deng J, Wang P, Yang M, Chen X, Zhu L, Liu J, Lu B, Shen Y, Fushimi K, Xu Q, Wu JY
Human molecular genetics 2016 Dec 1;25(23):5059-5068

---

FUS Interacts with HSP60 to Promote Mitochondrial Damage.

Deng J, Yang M, Chen Y, Chen X, Liu J, Sun S, Cheng H, Li Y, Bigio EH, Mesulam M, Xu Q, Du S, Fushimi K, Zhu L, Wu JY
PLoS genetics 2015 Sep;11(9):e1005357

---

Poly-A binding protein-1 localization to a subset of TDP-43 inclusions in amyotrophic lateral sclerosis occurs more frequently in patients harboring an expansion in C9orf72.

McGurk L, Lee VM, Trojanowksi JQ, Van Deerlin VM, Lee EB, Bonini NM
Journal of neuropathology and experimental neurology 2014 Sep;73(9):837-45

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Hu antigen R (HuR) is a positive regulator of the RNA-binding proteins TDP-43 and FUS/TLS: implications for amyotrophic lateral sclerosis.

Lu L, Zheng L, Si Y, Luo W, Dujardin G, Kwan T, Potochick NR, Thompson SR, Schneider DA, King PH
The Journal of biological chemistry 2014 Nov 14;289(46):31792-31804

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Topography of FUS pathology distinguishes late-onset BIBD from aFTLD-U.

Lee EB, Russ J, Jung H, Elman LB, Chahine LM, Kremens D, Miller BL, Branch Coslett H, Trojanowski JQ, Van Deerlin VM, McCluskey LF
Acta neuropathologica communications 2013 May 9;1(9):1-11

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The RNA-binding protein Fus directs translation of localized mRNAs in APC-RNP granules.

Yasuda K, Zhang H, Loiselle D, Haystead T, Macara IG, Mili S
The Journal of cell biology 2013 Dec 9;203(5):737-46

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Transportin 1 accumulates specifically with FET proteins but no other transportin cargos in FTLD-FUS and is absent in FUS inclusions in ALS with FUS mutations.

Neumann M, Valori CF, Ansorge O, Kretzschmar HA, Munoz DG, Kusaka H, Yokota O, Ishihara K, Ang LC, Bilbao JM, Mackenzie IR
Acta neuropathologica 2012 Nov;124(5):705-16

Western blot

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

K-562 cells were subjected to SDS PAGE followed by western blot with 60160-1-Ig(FUS antibody) at dilution of 1:1000

Sample type

cell line