NBP2-52874

antibody from Novus Biologicals

Targeting: FUS ALS6, FUS1, hnRNP-P2, HNRNPP2, TLS

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP2-52874

Provider

Novus Biologicals

Product name

Mouse Monoclonal FUS Antibody

Antibody type

Monoclonal

Description

Protein A purified. Specificity of human FUS antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.

Reactivity

Human

Host

Mouse

Antigen sequence

YGGQQQSYGQ


Isotype

IgG

Vial size

0.1 ml

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

References

Submitted references

PARP-1 Activation Directs FUS to DNA Damage Sites to Form PARG-Reversible Compartments Enriched in Damaged DNA.

Singatulina AS, Hamon L, Sukhanova MV, Desforges B, Joshi V, Bouhss A, Lavrik OI, Pastré D
Cell reports 2019 May 7;27(6):1809-1821.e5

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: FUS Antibody (CL0190) [NBP2-52874] - Analysis of extracts from U-251 cells, transfected with: control siRNA, target specific siRNA probe #1, target specific siRNA probe #2, using Anti-FUS monoclonal antibody. Downregulation of antibody signal confirms target specificity. Remaining intensity is presented. Loading control: Anti-GAPDH.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: FUS Antibody (CL0190) [NBP2-52874] - Lane 1: Marker [kDa], Lane 2: Human cell line HeLa cytoplasmic fraction , Lane 3: Human cell line HeLa membrane fraction , Lane 4: Human cell line HeLa nuclear fraction , Lane 5: Human cell line HeLa chromatin fraction , Lane 6: Human cell line HeLa cytos

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: FUS Antibody (CL0190) [NBP2-52874] - Lane 1: Marker [kDa] Lane 2: Human cell line HeLa Lane 3: Human cell line A-431 Lane 4: Human cell line MCF-7 Lane 5: Human cell line U2-OS Lane 6: Human cell line Hep-G2

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: FUS Antibody (CL0190) [NBP2-52874] - Staining of human endometrium shows strong nuclear positivity in glandular cells.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: FUS Antibody (CL0190) [NBP2-52874] - Staining of human cerebral cortex shows strong nuclear positivity in neurons.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: FUS Antibody (CL0190) [NBP2-52874] - Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: FUS Antibody (CL0190) [NBP2-52874] - Staining of human liver shows only very weak nuclear positivity in hepatocytes.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: FUS Antibody (CL0190) [NBP2-52874] - Staining in human cerebral cortex and liver tissues. Corresponding FUS RNA-seq data are presented for the same tissues.